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Optimization and Application of a Multiplex Digital PCR Assay for the Detection of SARS-CoV-2 Variants of Concern in Belgian Influent Wastewater
| Content Provider | MDPI |
|---|---|
| Author | Boogaerts, Tim Bogaert, Siel Van Den Poelvoorde, Laura A. E. Van Masri, Diala El Roeck, Naomi De Roosens, Nancy H. C. Lesenfants, Marie Lahousse, Lies Hoorde, Koenraad Van van Nuijs, Alexander L. N. Delputte, Peter |
| Copyright Year | 2022 |
| Description | Since the beginning of the COVID-19 pandemic, the wastewater-based epidemiology (WBE) of SARS-CoV-2 has been used as a complementary indicator to follow up on the trends in the COVID-19 spread in Belgium and in many other countries. To further develop the use of WBE, a multiplex digital polymerase chain reaction (dPCR) assay was optimized, validated and applied for the measurement of emerging SARS-CoV-2 variants of concern (VOC) in influent wastewater (IWW) samples. Key mutations were targeted in the different VOC strains, including SΔ69/70 deletion, N501Y, SΔ241 and SΔ157. The presented bioanalytical method was able to distinguish between SARS-CoV-2 RNA originating from the wild-type and B.1.1.7, B.1.351 and B.1.617.2 variants. The dPCR assay proved to be sensitive enough to detect low concentrations of SARS-CoV-2 RNA in IWW since the limit of detection of the different targets ranged between 0.3 and 2.9 copies/µL. This developed WBE approach was applied to IWW samples originating from different Belgian locations and was able to monitor spatio-temporal changes in the presence of targeted VOC strains in the investigated communities. The present dPCR assay developments were realized to bring added-value to the current national WBE of COVID-19 by also having the spatio-temporal proportions of the VoC in presence in the wastewaters. |
| Starting Page | 610 |
| e-ISSN | 19994915 |
| DOI | 10.3390/v14030610 |
| Journal | Viruses |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-03-15 |
| Access Restriction | Open |
| Subject Keyword | Viruses Analytical Chemistry Wastewater-based Epidemiology Variants of Concern Digital Polymerase Chain Reaction Belgium Sars-cov-2 |
| Content Type | Text |
| Resource Type | Article |
