NDLI: Metabolite Profiling of Methanolic Extract of Gardenia jaminoides by LC-MS/MS and GC-MS and Its Anti-Diabetic, and Anti-Oxidant Activities

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Metabolite Profiling of Methanolic Extract of Gardenia jaminoides by LC-MS/MS and GC-MS and Its Anti-Diabetic, and Anti-Oxidant Activities

Content Provider	MDPI
Author	Saravanakumar, Kandasamy Park, Seonju Sathiyaseelan, Anbazhagan Kim, Kil-Nam Cho, Su-Hyeon Mariadoss, Arokia Wang, Myeong-Hyeon
Copyright Year	2021
Description	In this study, the methanolic extract from seeds of Gardenia jasminoides exhibited strong antioxidant and enzyme inhibition activities with less toxicity to NIH3T3 and HepG2 cells at the concentration of 100 µg/mL. The antioxidant activities (DPPH and ABTS), α-amylase, and α-glucosidase inhibition activities were found higher in methanolic extract (MeOH-E) than H2O extract. Besides, 9.82 ± 0.62 µg and 6.42 ± 0.26 µg of MeOH-E were equivalent to 1 µg ascorbic acid for ABTS and DPPH scavenging, respectively while 9.02 ± 0.25 µg and 6.52 ± 0.15 µg of MeOH-E were equivalent to 1 µg of acarbose for inhibition of α-amylase and α-glucosidase respectively. Moreover, the cell assay revealed that the addition of MeOH-E (12.5 µg/mL) increased about 37% of glucose uptake in insulin resistant (IR) HepG2 as compared to untreated IR HepG2 cells. The LC- MS/MS and GC-MS analysis of MeOH-E revealed a total of 54 compounds including terpenoids, glycosides, fatty acid, phenolic acid derivatives. Among the identified compounds, chlorogenic acid and jasminoside A were found promising for anti-diabetic activity revealed by molecular docking study and these molecules are deserving further purification and molecular analysis.
Starting Page	102
e-ISSN	14248247
DOI	10.3390/ph14020102
Journal	Pharmaceuticals
Issue Number	2
Volume Number	14
Language	English
Publisher	MDPI
Publisher Date	2021-01-28
Access Restriction	Open
Subject Keyword	Pharmaceuticals Integrative and Complementary Medicine Gardenia Jasminoides Ellis Anti-diabetic Activity Lc-ms/ms Gc-ms Anti-oxidant
Content Type	Text
Resource Type	Article

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