NDLI: New Insights to Regulation of Fructose-1,6-bisphosphatase during Anoxia in Red-Eared Slider, Trachemys scripta elegans

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New Insights to Regulation of Fructose-1,6-bisphosphatase during Anoxia in Red-Eared Slider, Trachemys scripta elegans

Content Provider	MDPI
Author	Gupta, Aakriti Varma, Anchal Storey, Kenneth B.
Copyright Year	2021
Description	The red-eared slider (Trachemys scripta elegans) undergoes numerous changes to its physiological and metabolic processes to survive without oxygen. During anoxic conditions, its metabolic rate drops drastically to minimize energy requirements. The alterations in the central metabolic pathways are often accomplished by the regulation of key enzymes. The regulation of one such enzyme, fructose-1,6-bisphosphatase (FBPase; EC 3.1.3.11), was characterized in the present study during anoxia in liver. FBPase is a crucial enzyme of gluconeogenesis. The FBPase was purified from liver tissue in both control and anoxic conditions and subsequently assayed to determine the kinetic parameters of the enzyme. The study revealed the relative degree of post-translational modifications in the FBPase from control and anoxic turtles. Further, this study demonstrated a significant decrease in the maximal activity in anoxic FBPase and decreased sensitivity to its substrate Fructose-1,6-bisphosphate (FBP) when compared to the control. Immunoblotting demonstrated increased threonine phosphorylation (~1.4-fold) in the anoxic FBPase. Taken together, these results suggest that the phosphorylation of liver FBPase is an important step in suppressing FBPase activity, ultimately leading to the inhibition of gluconeogenesis in the liver of the red-eared slider during anaerobic conditions.
Starting Page	1548
e-ISSN	2218273X
DOI	10.3390/biom11101548
Journal	Biomolecules
Issue Number	10
Volume Number	11
Language	English
Publisher	MDPI
Publisher Date	2021-10-19
Access Restriction	Open
Subject Keyword	Biomolecules Trachemys Scripta Elegans Fructose-1,6-bisphosphatse Enzyme Regulation Gluconeogenesis Anoxia Metabolic Rate Depression Post-translational Modification
Content Type	Text
Resource Type	Article

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