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Optimisation of Xylanase–Pectinase Cocktail Production with Bacillus amyloliqiens ADI2 Using a Low-Cost Substrate via Statistical Strategy
| Content Provider | MDPI |
|---|---|
| Author | Norazliza, Sa’Ad Ainun, Zuriyati Asa’Ari Nawawi, Muhammad Hariadi Ismail, Khairul Izdihar Mohamad, Rosfarizan Tahir, Paridah Saad, Wan Zuhainis |
| Copyright Year | 2022 |
| Description | An effective statistical tool for increasing and boosting the production of xylanase and pectinase by Bacillus amyloliqiens ADI2 during submerged fermentation (SmF) appears to be the response of surface methodology (RSM) using the central composite design (CCD). Optimum production was achieved under fermentation conditions of a temperature of 28 °C, pH of 8.38, inoculum size of 4% (w/v) and agitation speed of 94 rpm for 48 h. The experimental responses demonstrated a near agreement with the expected responses under optimum conditions of independent variables, suggesting the model’s validity. The optimised CCD model had a 1.34-fold, 159 ± 6 U/mL greater xylanase and 5.96-fold, 205 ± 9 U/mL greater pectinase production than the one factor at a time (OFAT) approach. The production of concurrent enzymes of xylanase–pectinase resulted in a ratio of 1:1.3. |
| Starting Page | 119 |
| e-ISSN | 23115637 |
| DOI | 10.3390/fermentation8030119 |
| Journal | Fermentation |
| Issue Number | 3 |
| Volume Number | 8 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-03-10 |
| Access Restriction | Open |
| Subject Keyword | Fermentation Microbiology Rsm Xylanase Pectinase Bacillus Production |
| Content Type | Text |
| Resource Type | Article |