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Critical Optimized Conditions for Gamma-Aminobutyric Acid (GABA)-Producing Tetragenococcus Halophilus Strain KBC from a Commercial Soy Sauce Moromi in Batch Fermentation
| Content Provider | MDPI |
|---|---|
| Author | Sassi, Soumaya Ilham, Zul Jamaludin, Nazzatush Shimar Halim-Lim, Sarina Abdul Yee, Chong Shin Loen, Alan Wong Weng Suan, Ooi Poh Ibrahim, Mohamad Faizal Wan-Mohtar, Wan Abd Al Qadr Imad |
| Copyright Year | 2022 |
| Description | Gamma-aminobutyric acid (GABA) has several health-promoting qualities, leading to a growing demand for natural GABA production via microbial fermentation. The GABA-producing abilities of the new Tetragenococcus halophilus (THSK) isolated from a commercial soy sauce moromi were proven in this investigation. Under aerobic conditions, the isolate produced 293.43 mg/L of GABA after 5 days of cultivation, compared to 217.13 mg/L under anaerobic conditions. Critical parameters such as pH, monosodium glutamate (MSG), and sodium chloride (NaCl) concentrations were examined to improve GABA yield. MSG had the most significant impact on GABA and GABA synthesis was not suppressed even at high NaCl concentrations. Data showed that a pH of 8, MSG content of 5 g/L, and 20% NaCl were the best culture conditions. The ultimate yield was improved to 653.101 mg/L, a 2.22-fold increase (293.43 mg/L). This design shows that the bacteria THSK has industrial GABA production capability and can be incorporated into functional food. |
| Starting Page | 409 |
| e-ISSN | 23115637 |
| DOI | 10.3390/fermentation8080409 |
| Journal | Fermentation |
| Issue Number | 8 |
| Volume Number | 8 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-08-19 |
| Access Restriction | Open |
| Subject Keyword | Fermentation Microbiology Soy Sauce Moromi Gaba Monosodium Glutamate Tetragenococcus Halophilus Future Food |
| Content Type | Text |
| Resource Type | Article |
