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High-Light Versus Low-Light: Effects on Paired Photosystem II Supercomplex Structural Rearrangement in Pea Plants
| Content Provider | MDPI |
|---|---|
| Author | Grinzato, Alessandro Albanese, Pascal Marotta, Roberto Swuec, Paolo Saracco, Guido Bolognesi, Martino Zanotti, Giuseppe Pagliano, Cristina |
| Copyright Year | 2020 |
| Description | In plant grana thylakoid membranes Photosystem II (PSII) associates with a variable number of antenna proteins (LHCII) to form different types of supercomplexes (PSII-LHCII), whose organization is dynamically adjusted in response to light cues, with the C2S2 more abundant in high-light and the C2S2M2 in low-light. Paired PSII-LHCII supercomplexes interacting at their stromal surface from adjacent thylakoid membranes were previously suggested to mediate grana stacking. Here, we present the cryo-electron microscopy maps of paired C2S2 and C2S2M2 supercomplexes isolated from pea plants grown in high-light and low-light, respectively. These maps show a different rotational offset between the two supercomplexes in the pair, responsible for modifying their reciprocal interaction and energetic connectivity. This evidence reveals a different way by which paired PSII-LHCII supercomplexes can mediate grana stacking at diverse irradiances. Electrostatic stromal interactions between LHCII trimers almost completely overlapping in the paired C2S2 can be the main determinant by which PSII-LHCII supercomplexes mediate grana stacking in plants grown in high-light, whereas the mutual interaction of stromal N-terminal loops of two facing Lhcb4 subunits in the paired C2S2M2 can fulfil this task in plants grown in low-light. The high-light induced accumulation of the Lhcb4.3 protein in PSII-LHCII supercomplexes has been previously reported. Our cryo-electron microscopy map at 3.8 Å resolution of the C2S2 supercomplex isolated from plants grown in high-light suggests the presence of the Lhcb4.3 protein revealing peculiar structural features of this high-light-specific antenna important for photoprotection. |
| Starting Page | 8643 |
| e-ISSN | 14220067 |
| DOI | 10.3390/ijms21228643 |
| Journal | International Journal of Molecular Sciences |
| Issue Number | 22 |
| Volume Number | 21 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2020-11-16 |
| Access Restriction | Open |
| Subject Keyword | International Journal of Molecular Sciences Plant Sciences Cryo-electron Microscopy Light Acclimation Photosystem Ii Supercomplex Plant Thylakoid Membranes |
| Content Type | Text |
| Resource Type | Article |
