NDLI: Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust

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Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust

Content Provider	MDPI
Author	Zhang, Jie Tang, Shuyao Lang, Tao Wang, Ying Long, Hai Deng, Guangbing Chen, Qian Guo, Yuanlin Xuan, Pu Xiao, Jun Jiang, Yun
Copyright Year	2022
Description	The annual species Dasypyrum villosum possesses several potentially valuable genes for the improvement of common wheat. Previously, we identified a new stripe rust-resistant line, the Chinese Spring (CS)–D. villosum 3V#3 (3D) substitution line (named CD-3), and mapped its potential rust resistance gene (designated as YrCD-3) on the 3V#3 chromosome originating from D. villosum. The objective of the present study was to further narrow down the YrCD-3 locus to a physical region and develop wheat-3V#3 introgression lines with strong stripe rust resistance. By treating CD-3 seeds with$ ^{60}$Co γ-irradiation, two CS-3V#3 translocation lines, T3V#3S.3DL and T3DS.3V#3L (termed 22-12 and 24-20, respectively), were identified from the $M_{4}$ generation through a combination of non-denaturing fluorescence in situ hybridization (ND-FISH) and functional molecular markers. Stripe rust resistance tests showed that the line 22-12 exhibited strong stripe rust resistance similarly to CD-3, whereas 24-20 was susceptible to stripe rust similarly to CS, indicating that YrCD-3 is located on the short arm of 3V#3. The line 22-12 can potentially be used for further wheat improvement. Additionally, to trace 3V#3 in the wheat genetic background, we produced 30 3V#3-specific sequence tag (EST) markers, among which, 11 markers could identify 3V#3S. These markers could be valuable in fine-mapping YrCD-3.
Starting Page	1329
e-ISSN	22237747
DOI	10.3390/plants11101329
Journal	Plants
Issue Number	10
Volume Number	11
Language	English
Publisher	MDPI
Publisher Date	2022-05-18
Access Restriction	Open
Subject Keyword	Plants Agronomy D. Villosum Translocation Line Stripe Rust Molecular Marker Nd-fish
Content Type	Text
Resource Type	Article

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