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An Identification System Targeting the SRK Gene for Selecting S-Haplotypes and Self-Compatible Lines in Cabbage
| Content Provider | MDPI |
|---|---|
| Author | Chen, Wendi Zhang, Bin Ren, Wenjing Chen, Li Fang, Zhiyuan Yang, Limei Zhuang, Mu Lv, Honghao Wang, Yong Zhang, Yangyong |
| Copyright Year | 2022 |
| Description | Cabbage (Brassica oleracea L. var. capitata) self-incompatibility is important for heterosis. However, the seed production of elite hybrid cannot be facilitated by honey bees due to the cross-incompatibility of the two parents. In this study, the self-compatibility of 58 winter cabbage inbred lines was identified by open-flower self-pollination (OS) and molecular techniques. Based on the NCBI database, a new class I S-haplotype-specific marker, PKC6F/PKC6R, was developed. Verification analyses revealed 9 different S-haplotypes in the 58 cabbage inbred lines; of these lines, 46 and 12 belonged to class I (S6, S7, S12, S14, S33, S45, S51, S68) and class II (S15) S-haplotypes, respectively. The coincidence rate between the self-compatibility index and S-haplotype was 91%. This study developed a Tri-Primer-PCR amplification method to rapidly select plants with specific S-haplotypes in biased segregated S-locus populations. Furthermore, it established an S-haplotype identification system based on these nine S-haplotypes. To overcome parental cross-incompatibility (18-503 and 18-512), an inbred line (18-2169) with the S15 haplotype was selected from the sister lines of self-incompatible 18-512 (S68, class I S-haplotype). The inbred line (18-2169) showed self-compatibility and cross-compatibility with 18-503. This study provides guidance for self-compatibility breeding in cabbage and predicts parental cross-incompatibility in elite combinations. |
| Starting Page | 1372 |
| e-ISSN | 22237747 |
| DOI | 10.3390/plants11101372 |
| Journal | Plants |
| Issue Number | 10 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-05-21 |
| Access Restriction | Open |
| Subject Keyword | Plants Horticulture Cabbage Self-incompatibility S-haplotype Primer Set Cross-incompatibility |
| Content Type | Text |
| Resource Type | Article |
