NDLI: Discrimination between the Two Closely Related Species of the Operational Group B. amyloliqiens Based on Whole-Cell Fatty Acid Profiling

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Discrimination between the Two Closely Related Species of the Operational Group B. amyloliqiens Based on Whole-Cell Fatty Acid Profiling

Content Provider	MDPI
Author	Csaba, Vágvölgyi Vörös, Mónika Huynh, Thu Kedves, Orsolya Turbat, Adiyadolgor Sipos, György Leitgeb, Balázs Kredics, László Szekeres, András
Copyright Year	2022
Description	(1) Background: Bacillus velezensis and Bacillus amyloliqiens are closely related members of the “operational group B. amyloliqiens”, a taxonomical unit above species level within the ”Bacillus subtilis species complex”. They have similar morphological, physiological, biochemical, phenotypic, and phylogenetic characteristics. Thus, separating these two taxa from each another has proven to be difficult to implement and could not be pushed easily into the line of routine analyses. (2) Methods: The aim of this study was to determine whether whole FAME profiling could be used to distinguish between these two species, using both type strains and environmental isolates. Initially, the classification was determined by partial sequences of the gyrA and rpoB genes and the classified isolates and type strains were considered as samples to develop the identification method, based on FAME profiles. (3) Results: The dissimilarities in 16:0, 17:0 iso, and 17:0 FA components have drawn a distinction between the two species and minor differences in FA 14:0, 15:0 iso, and 16:0 iso were also visible. The statistical analysis of the FA profiles confirmed that the two taxa can be distinguished into two separate groups, where the isolates are identified without misreading. (4) Conclusions: Our study proposes that the developed easy and fast-automated identification tool based on cellular FA profiles can be routinely applied to distinguish B. velezensis and B. amyloliqiens.
Starting Page	418
e-ISSN	20762607
DOI	10.3390/microorganisms10020418
Journal	Microorganisms
Issue Number	2
Volume Number	10
Language	English
Publisher	MDPI
Publisher Date	2022-02-11
Access Restriction	Open
Subject Keyword	Microorganisms Microbiology Bacillus Taxonomy Bacillus Velezensis Bacillus Amyloliquefaciens Fatty Acid Profiling Chemotaxonomy
Content Type	Text
Resource Type	Article

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