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Heterologous Expression of Full-Length and Truncated Human ZIP4 Zinc Transporter in Saccharomyces cerevisiae
| Content Provider | MDPI |
|---|---|
| Author | Liu, Yuting Bafaro, Elizabeth M. Dempski, Robert E. |
| Copyright Year | 2022 |
| Description | The human (h) transporter hZIP4 is the primary $Zn^{2+}$ importer in the intestine. hZIP4 is also expressed in a variety of organs such as the pancreas and brain. Dysfunction of hZIP4 can result in the $Zn^{2+}$ deficiency disease acrodermatitis enteropathica (AE). AE can disrupt digestive and immune system homeostasis. A limited number of hZIP4 expression strategies have hindered increasing knowledge about this essential transmembrane protein. Here, we report the heterologous expression of hZIP4 in Saccharomyces cerevisiae. Both a wild-type and a mutant S. cerevisiae strain, in which the endogenous $Zn^{2+}$ transporters were deleted, were used to test the expression and localization of an hZIP4–GFP fusion protein. A full-length hZIP4–GFP and a truncated membrane-domain-only (mhZIP4–GFP) protein were observed to be present in the plasma membrane in yeast. |
| Starting Page | 726 |
| e-ISSN | 2218273X |
| DOI | 10.3390/biom12050726 |
| Journal | Biomolecules |
| Issue Number | 5 |
| Volume Number | 12 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-05-21 |
| Access Restriction | Open |
| Subject Keyword | Biomolecules Immunology Human Zinc Transporter Membrane Protein Heterologous Expression in Yeast Gfp Fusion Protein |
| Content Type | Text |
| Resource Type | Article |
