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The Nuts and Bolts of SARS-CoV-2 Spike Receptor-Binding Domain Heterologous Expression
| Content Provider | MDPI |
|---|---|
| Author | Maffei, Mariano Montemiglio, Linda Celeste Vitagliano, Grazia Fedele, Luigi Sellathurai, Shaila Bucci, Federica Compagnone, Mirco Chiarini, Valerio Exertier, Cécile Muzi, Alessia Roscilli, Giuseppe Vallone, Beatrice Marra, Emanuele |
| Copyright Year | 2021 |
| Description | COVID-19 is a highly infectious disease caused by a newly emerged coronavirus (SARS-CoV-2) that has rapidly progressed into a pandemic. This unprecedent emergency has stressed the significance of developing effective therapeutics to fight the current and future outbreaks. The receptor-binding domain (RBD) of the SARS-CoV-2 surface Spike protein is the main target for vaccines and represents a helpful “tool” to produce neutralizing antibodies or diagnostic kits. In this work, we provide a detailed characterization of the native RBD produced in three major model systems: Escherichia coli, insect and HEK-293 cells. Circular dichroism, gel filtration chromatography and thermal denaturation experiments indicated that recombinant SARS-CoV-2 RBD proteins are stable and correctly folded. In addition, their functionality and receptor-binding ability were further evaluated through ELISA, flow cytometry assays and bio-layer interferometry. |
| Starting Page | 1812 |
| e-ISSN | 2218273X |
| DOI | 10.3390/biom11121812 |
| Journal | Biomolecules |
| Issue Number | 12 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-12-02 |
| Access Restriction | Open |
| Subject Keyword | Biomolecules Virology Sars-cov-2 Receptor-binding Domain Covid-19 Spike Protein Heterologous Expression Protein Production |
| Content Type | Text |
| Resource Type | Article |
