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A Bibliometric Analysis and Review of Pullulan-Degrading Enzymes—Past and Current Trends
| Content Provider | MDPI |
|---|---|
| Author | Kahar, Ummirul Mukminin Latif, Nurriza Ab Amran, Syazwani Itri Liew, Kok Jun Goh, Kian Mau |
| Copyright Year | 2022 |
| Description | Starch and pullulan degrading enzymes are essential industrial biocatalysts. Pullulan-degrading enzymes are grouped into pullulanases (types I and type II) and pullulan hydrolase (types I, II and III). Generally, these enzymes hydrolyse the α-1,6 glucosidic bonds (and α-1,4 for certain enzyme groups) of substrates and form reducing sugars such as glucose, maltose, maltotriose, panose or isopanose. This review covers two main aspects: (i) bibliometric analysis of publications and patents related to pullulan-degrading enzymes and (ii) biological aspects of free and immobilised pullulan-degrading enzymes and protein engineering. The collective data suggest that most publications involved researchers within the same institution or country in the past and current practice. Multi-national interaction shall be improved, especially in tapping the enzymes from unculturable prokaryotes. While the understanding of pullulanases may reach a certain extend of saturation, the discovery of pullulan hydrolases is still limited. In this report, we suggest readers consider using the next-generation sequencing technique to fill the gaps of finding more new sequences encoding pullulan-degrading enzymes to expand the knowledge body of this topic. |
| Starting Page | 143 |
| e-ISSN | 20734344 |
| DOI | 10.3390/catal12020143 |
| Journal | Catalysts |
| Issue Number | 2 |
| Volume Number | 12 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-01-24 |
| Access Restriction | Open |
| Subject Keyword | Catalysts Microbiology Amylopullulanase Alpha-amylase-pullulanase Carbohydrate-active Enzyme Glycoside Hydrolase Isopullulanase Neopullulanase Pullulan Pullulan Hydrolase Starch Type I Pullulanase |
| Content Type | Text |
| Resource Type | Article |