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Performance of the $LIAISON^{®}$ SARS-CoV-2 Antigen Assay vs. SARS-CoV-2-RT-PCR
| Content Provider | MDPI |
|---|---|
| Author | Fiedler, Melanie Holtkamp, Caroline Dittmer, Ulf Anastasiou, Olympia |
| Copyright Year | 2021 |
| Description | We aimed to evaluate the $LIAISON^{®}$ SARS-CoV-2 antigen assay (DiaSorin), comparing its performance to real-time polymerase chain reaction (RT-PCR) for the detection of SARS-CoV-2 RNA. 182 (110 PCR-positive and 72 PCR-negative) nasopharyngeal swab samples were taken for the detection of SARS-CoV-2. RT-PCR and antigen assay were performed using the same material. The sensitivity and specificity of the antigen assay were calculated for different cut-offs, with RT-PCR serving as the reference method. Stored clinical samples that were positive for other respiratory viruses were tested to evaluate cross-reactivity. One third (33/110, 30%) were falsely classified as negative, while no false positives were found using the 200 $TCID_{50}$/mL cut-off for the SARS-CoV-2 antigen as proposed by the manufacturer. This corresponded to a sensitivity of 70% (60–78%) and a specificity of 100% (94–100%). Lowering the cut-off for positivity of the antigen assay to 22.79 or 57.68 $TCID_{50}$/mL increased the sensitivity of the method, reaching a sensitivity of 92% (85–96%) vs. 79% (70–86%) and a specificity of 81% (69–89%) vs. 99% (91–100%), respectively. The antigen assay reliably detected samples with high SARS-CoV-2 viral loads $(≥10^{6}$ copies SARS-CoV-2/mL), while it cannot differentiate between negative and low positive samples. Cross-reactivity toward other respiratory viruses was not detected. |
| Starting Page | 658 |
| e-ISSN | 20760817 |
| DOI | 10.3390/pathogens10060658 |
| Journal | Pathogens |
| Issue Number | 6 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-05-26 |
| Access Restriction | Open |
| Subject Keyword | Pathogens Microbiology Sars-cov-2 Covid-19 Antigen Liaison® Sars-cov-2 Antigen Assay Rt-pcr |
| Content Type | Text |
| Resource Type | Article |
