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Concatenation of Transgenic DNA: Random or Orchestrated?
| Content Provider | MDPI |
|---|---|
| Author | Smirnov, Alexander Battulin, Nariman |
| Copyright Year | 2021 |
| Description | Generation of transgenic organisms by pronuclear microinjection has become a routine procedure. However, while the process of DNA integration in the genome is well understood, we still do not know much about the recombination between transgene molecules that happens in the first moments after DNA injection. Most of the time, injected molecules are joined together in head-to-tail tandem repeats—the so-called concatemers. In this review, we focused on the possible concatenation mechanisms and how they could be studied with genetic reporters tracking individual copies in concatemers. We also discuss various features of concatemers, including palindromic junctions and repeat-induced gene silencing (RIGS). Finally, we speculate how cooperation of DNA repair pathways creates a multicopy concatenated insert. |
| Starting Page | 1969 |
| e-ISSN | 20734425 |
| DOI | 10.3390/genes12121969 |
| Journal | Genes |
| Issue Number | 12 |
| Volume Number | 12 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-12-10 |
| Access Restriction | Open |
| Subject Keyword | Genes Cell Biology Transgenic Animals Concatemers Double-strand Breaks (dsbs) Homologous Recombination (hr) Non-homologous End-joining (nhej) Palindromes Repeat-induced Gene Silencing (rigs) Pronuclear Microinjection |
| Content Type | Text |
| Resource Type | Article |
