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Thin Film Plastic Antibody-Based Microplate Assay for Human Serum Albumin Determination
| Content Provider | MDPI |
|---|---|
| Author | Boonsriwong, Worachote Chunta, Suticha Thepsimanon, Nonthawat Singsanan, Sanita Lieberzeit, Peter |
| Copyright Year | 2021 |
| Description | Herein we demonstrate molecularly imprinted polymers (MIP) as plastic antibodies for a microplate-based assay. As the most abundant plasma protein, human serum albumin (HSA) was selected as the target analyte model. Thin film MIP was synthesized by the surface molecular imprinting approach using HSA as the template. The optimized polymer consisted of acrylic acid (AA) and N-vinylpyrrolidone (VP) in a 2:3 (w/w) ratio, crosslinked with N,N′-(1,2-dihydroxyethylene) bisacrylamide (DHEBA) and then coated on the microplate well. The binding of MIP toward the bound HSA was achieved via the Bradford reaction. The assay revealed a dynamic detection range toward HSA standards in the clinically relevant 1–10 g/dL range, with a 0.01 g/dL detection limit. HSA-MIP showed minimal interference from other serum protein components: γ-globulin had 11% of the HSA response, α-globulin of high-density lipoprotein had 9%, and β-globulin of low-density lipoprotein had 7%. The analytical accuracy of the assay was 89–106% at the 95% confidence interval, with precision at 4–9%. The MIP-coated microplate was stored for 2 months at room temperature without losing its binding ability. The results suggest that the thin film plastic antibody system can be successfully applied to analytical/pseudoimmunological HSA determinations in clinical applications. |
| Starting Page | 1763 |
| e-ISSN | 20734360 |
| DOI | 10.3390/polym13111763 |
| Journal | Polymers |
| Issue Number | 11 |
| Volume Number | 13 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-05-27 |
| Access Restriction | Open |
| Subject Keyword | Polymers Analytical Chemistry Human Serum Albumin Thin Film Mip-based Microplate Assay Molecularly Imprinted Polymer Plastic Antibody |
| Content Type | Text |
| Resource Type | Article |
