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Determination of the Phenolic Profile by Liquid Chromatography, Evaluation of Antioxidant Activity and Toxicity of Moroccan Erica multiflora, Erica scoparia, and Calluna vulgaris (Ericaceae)
Content Provider | MDPI |
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Author | Emilia, Cavò Bekkai, Douaa Majdoub, Yassine Oulad El Bekkai, Hamid Cacciola, Francesco Miceli, Natalizia Taviano, Maria Fernanda Errabii, Tomader Vinci, Roberto Laganà Mondello, Luigi Kbiach, Mohammed L’Bachir El |
Copyright Year | 2022 |
Description | This study aimed to investigate the phenolic profile and selected biological activities of the leaf and aerial extracts of three Ericaceae species, namely Erica multiflora, Erica scoparia, and Calluna vulgaris, collected from three different places in the north of Morocco. The phenolic composition of all extracts was determined by LC coupled with photodiode array and mass spectrometry detection. Among the investigated extracts, that of E. scoparia aerial parts was the richest one, with a total amount of polyphenols of 9528.93 mg/kg. Up to 59 phenolic compounds were detected: 52 were positively identified and 49 quantified—11 in C. vulgaris, 14 in E. multiflora, and 24 in E. scoparia. In terms of chemical classes, nine were phenolic acids and 43 were flavonoids, and among them, the majority belonged to the class of flavonols. The antioxidant activity of all extracts was investigated by three different in vitro methods, namely DPPH, reducing power, and $Fe^{2+}$ chelating assays; E. scoparia aerial part extract was the most active, with an $IC_{50}$ of 0.142 ± 0.014 mg/mL (DPPH test) and 1.898 ± 0.056 ASE/mL (reducing power assay). Further, all extracts were non-toxic against Artemia salina, thus indicating their potential safety. The findings attained in this work for such Moroccan Ericaceae species, never investigated so far, bring novelty to the field and show them to be valuable sources of phenolic compounds with interesting primary antioxidant properties. |
Starting Page | 3979 |
e-ISSN | 14203049 |
DOI | 10.3390/molecules27133979 |
Journal | Molecules |
Issue Number | 13 |
Volume Number | 27 |
Language | English |
Publisher | MDPI |
Publisher Date | 2022-06-21 |
Access Restriction | Open |
Subject Keyword | Molecules Integrative and Complementary Medicine Ericaceae Lc–dad/esi–ms Phenolic Compounds Flavonoids Antioxidant Activity Artemia Salina Leach |
Content Type | Text |
Resource Type | Article |