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Production of Carotenoids and Phospholipids by Thraustochytrium sp. in Batch and Repeated-Batch Culture
| Content Provider | MDPI |
|---|---|
| Author | Leyton, Allison Shene, Carolina Chisti, Yusuf Asenjo, Juan A. |
| Copyright Year | 2022 |
| Description | The carotenogenic thraustochytrid Thraustochytrium sp. RT2316-16 was grown in batch and repeated-batch cultures using different feeds containing glucose, or glycerol, and yeast extract, for the production of lipids, phospholipids and carotenoids. RT2316-16 produced canthaxanthin, astaxanthin and β-carotene. The effects of biotin, ascorbic acid, light and temperature were evaluated in some of the experiments. In 2-day-old batch cultures, the combined mass percentage of eicosapentaenoic acid and docosahexaenoic acid in total lipids was between 16.5% (glycerol-based medium in the dark; biomass concentration = 4.2 ± 1.1 g $L^{−1}$) and 42.6% (glucose-based medium under light; biomass concentration = 3.3 ± 0.1 g $L^{−1}$), decreasing to 3.8% and 6.1%, respectively, after day 4. In repeated-batch cultures, the total lipids in the biomass increased after glucose or glycerol was fed alone, whereas the total carotenoids (168 ± 7 μg $g^{−1}$ dry weight (DW)) and phospholipids in the biomass increased after feeding with yeast extract. The biomass with the highest content of phospholipids (28.7 ± 4.3 mg $g^{−1}$ DW) was obtained using a feed medium formulated with glycerol, yeast extract and ascorbic acid. Glycerol was the best carbon source for the production of a biomass enriched with total lipids (467 ± 45 mg $g^{−1}$ DW). The composition of carotenoids depended strongly on the composition of the feed. Repeated-batch cultures fed with yeast extract contained canthaxanthin as the main carotenoid, whereas in the cultures fed only with glucose, the biomass contained mainly β-carotene. |
| Starting Page | 416 |
| e-ISSN | 16603397 |
| DOI | 10.3390/md20070416 |
| Journal | Marine drugs |
| Issue Number | 7 |
| Volume Number | 20 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-06-25 |
| Access Restriction | Open |
| Subject Keyword | Marine drugs Marine Drugs Microbiology Thraustochytrium Sp. Thraustochytrids Carotenoids Canthaxanthin Docosahexaenoic Acid Phospholipids |
| Content Type | Text |
| Resource Type | Article |
