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Role of Lactic Acid Bacteria Phospho-β-Glucosidases during the Fermentation of Cereal by-Products
| Content Provider | MDPI |
|---|---|
| Author | Acin-Albiac, Marta Filannino, Pasquale Arora, Kashika Ros, Alessio Da Gobbetti, Marco Cagno, Raffaella Di |
| Copyright Year | 2021 |
| Description | Bioprocessing using lactic acid bacteria (LAB) is a powerful means to exploit plant-derived by-products as a food ingredient. LAB have the capability to metabolize a large variety of carbohydrates, but such metabolism only relies on few metabolic routes, conferring on them a high fermentation potential. One example of these pathways is that involving phospho-β-glucosidase genes, which are present in high redundancy within LAB genomes. This enzymatic activity undertakes an ambivalent role during fermentation of plant-based foods related to the release of a wide range of phenolic compounds, from their β-D-glycosylated precursors and the degradation of β-glucopyranosyl derived carbohydrates. We proposed a novel phenomic approach to characterize the metabolism drift of Lactiplantibacillus plantarum and Leuconostoc pseudomesenteroides caused by a lignocellulosic by-product, such as the brewers’ spent grain (BSG), in contrast to Rich De Man, Rogosa and Sharpe (MRS) broth. We observed an increased metabolic activity for gentiobiose, cellobiose and β-glucoside conjugates of phenolic compounds during BSG fermentation. Gene expression analysis confirmed the importance of cellobiose metabolism while a release of lignin-derived aglycones was found during BSG fermentation. We provided a comprehensive view of the important role exerted by LAB 6-phospho-β-glucosidases as well the major metabolic routes undertaken during plant-based fermentations. Further challenges will consider a controlled characterization of pbg gene expression correlated to the metabolism of β-glucosides with different aglycone moieties. |
| Starting Page | 97 |
| e-ISSN | 23048158 |
| DOI | 10.3390/foods10010097 |
| Journal | Foods |
| Issue Number | 1 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-01-05 |
| Access Restriction | Open |
| Subject Keyword | Foods Biochemical Research Phospho-beta-glucosidases Cereal By-products Brewers' Spent Grain Lactic Acid Bacteria Metabolism |
| Content Type | Text |
| Resource Type | Article |
