NDLI: In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms

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In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms

Content Provider	MDPI
Author	Jimmy, Hernández-Gómez Argelia, López-Bonilla Sandra, Ávila-Reyes Antonio, Jiménez-Aparicio Humberto, Hernández-Sánchez Trejo-Tapia, Gabriela
Copyright Year	2021
Description	Bile salt hydrolase (BSH) activity in probiotic strains is usually correlated with the ability to lower serum cholesterol levels in hypercholesterolemic patients. The objective of this study was the evaluation of BSH in five probiotic strains of lactic acid bacteria (LAB) and a probiotic yeast. The activity was assessed using a qualitative direct plate test and a quantitative high-performance thin- layer chromatography assay. The six strains differed in their BSH substrate preference and activity. Lactobacillus plantarum DGIA1, a potentially probiotic strain isolated from a double cream cheese from Chiapas, Mexico, showed excellent deconjugation activities in the four tested bile acids (69, 100, 81, and 92% for sodium glycocholate, glycodeoxycholate, taurocholate, and taurodeoxycholate, respectively). In the case of the commercial probiotic yeast Saccharomyces boulardii, the deconjugation activities were good against sodium glycodeoxycholate, taurocholate, and taurodeoxycholate (100, 57, and 63%, respectively). These last two results are part of the novelty of the work. A weak deconjugative activity (5%) was observed in the case of sodium glycocholate. This is the first time that the BSH activity has been detected in this yeast.
Starting Page	674
e-ISSN	23048158
DOI	10.3390/foods10030674
Journal	Foods
Issue Number	3
Volume Number	10
Language	English
Publisher	MDPI
Publisher Date	2021-03-22
Access Restriction	Open
Subject Keyword	Foods Microbiology Bile Salt Hydrolase Probiotics Lactobacillus Plantarum Saccharomyces Boulardii Cholesterol
Content Type	Text
Resource Type	Article

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