NDLI: Formulation of Piperine Nanoparticles: In Vitro Breast Cancer Cell Line and In Vivo Evaluation

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Formulation of Piperine Nanoparticles: In Vitro Breast Cancer Cell Line and In Vivo Evaluation

Content Provider	MDPI
Author	Kazmi, Imran Al-Abbasi, Fahad A. Imam, Syed Sarim Afzal, Muhammad Nadeem, Muhammad Shahid Altayb, Hisham N. Alshehri, Sultan
Copyright Year	2022
Description	Piperine (PPN), one of the most investigated phytochemicals, is known to have excellent therapeutic efficacy against a variety of ailments including breast cancer. However, its physicochemical properties such as poor aqueous solubility restrict its clinical application. Therefore, the present investigation was designed to develop PPN encapsulated lipid polymer hybrid nanoparticles (PPN-LPHNPs) to overcome the limitation. The developed PPN-LPHNPs were optimized by the three-factor, three-level Box–Behnken design $(3^{3}$-BBD). The optimized PPN-LPHNPs were then evaluated for their drug release profile, cytotoxicity assay against MDA-MB-231 and MCF-7 cells, and gastrointestinal stability as well as colloidal stability. In addition, the optimized PPN-LPHNPs were evaluated for ex vivo intestinal permeation and in vivo pharmacokinetic in albino Wistar rats. As per the results, the optimized PPN-LPHNPs showed a small average particles size of <160 nm with a low (+20 mV). PPN-LPHNPs revealed excellent gastrointestinal as well as colloidal stability and sustained release profiles up to 24 h. Furthermore, PPN-LPHNPs revealed excellent cytotoxicity against both MDA-MB-231 and MCF-7 cancer cells compared to the free PPN. Moreover, animal studies revealed that the PPN-LPHNPs exhibited a 6.02- and 4.55-fold higher intestinal permeation and relative oral bioavailability, respectively, in comparison to the conventional PPN suspension. Thus, our developed LPHNPs present a strong potential for improved delivery of PPN.
Starting Page	1349
e-ISSN	20734360
DOI	10.3390/polym14071349
Journal	Polymers
Issue Number	7
Volume Number	14
Language	English
Publisher	MDPI
Publisher Date	2022-03-26
Access Restriction	Open
Subject Keyword	Polymers Toxicology Box–behnken Design Chitosan Mucoadhesion Breast Cancer Intestinal Permeation Oral Bioavailability
Content Type	Text
Resource Type	Article

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