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Photolytic Measurement of Tissue S-Nitrosothiols in Rats and Humans In Vivo
| Content Provider | MDPI |
|---|---|
| Author | Neidigh, Noah Alexander, Alyssa van Emmerik, Parker Higgs, Allison Plack, Logan Clem, Charles Cater, Daniel Marozkina, Nadzeya Gaston, Benjamin |
| Copyright Year | 2022 |
| Description | S-nitrosothiols are labile thiol-NO adducts formed in vivo primarily by metalloproteins such as NO synthase, ceruloplasmin, and hemoglobin. Abnormal S-nitrosothiol synthesis and catabolism contribute to many diseases, ranging from asthma to septic shock. Current methods for quantifying S-nitrosothiols in vivo are suboptimal. Samples need to be removed from the body for analysis, and the S-nitrosothiols can be broken down during ex vivo processing. Here, we have developed a noninvasive device to measure mammalian tissue S-nitrosothiols in situ non-invasively using ultraviolet (UV) light, which causes NO release in proportion to the S-nitrosothiol concentration. We validated the assay in vitro; then, we applied it to measure S-nitrosothiols in vivo in rats and in humans. The method was sensitive to 0.5 µM, specific (did not detect other nitrogen oxides), and was reproducible in rats and in humans. This noninvasive approach to S-nitrosothiol measurements may be applicable for use in human diseases. |
| Starting Page | 1294 |
| e-ISSN | 14203049 |
| DOI | 10.3390/molecules27041294 |
| Journal | Molecules |
| Issue Number | 4 |
| Volume Number | 27 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-02-15 |
| Access Restriction | Open |
| Subject Keyword | Molecules S-nitrosothiol Photolytic Cleavage Ultraviolet Light Nitric Oxide Noninvasive Measurements |
| Content Type | Text |
| Resource Type | Article |
