NDLI: Development of a Tet-On Inducible Expression System for the Anhydrobiotic Cell Line, Pv11

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Development of a Tet-On Inducible Expression System for the Anhydrobiotic Cell Line, Pv11

Content Provider	MDPI
Author	Tokumoto, Shoko Miyata, Yugo Usui, Kengo Deviatiiarov, Ruslan Ohkawa, Takahiro Kondratieva, Sabina Shagimardanova, Elena Gusev, Oleg Cornette, Richard Itoh, Masayoshi Hayashizaki, Yoshihide Kikawada, Takahiro
Copyright Year	2020
Description	The Pv11 cell line established from an African chironomid, Polypedilum vanderplanki, is the only cell line tolerant to complete desiccation. In Pv11 cells, a constitutive expression system for Pv11 cells was previously exploited and several reporter genes were successfully expressed. Here we report the identification of an effective minimal promoter for Pv11 cells and its application to the Tet-On inducible expression system. First, using a luciferase reporter assay, we showed that a 202 bp deletion fragment derived from the constitutively active 121-promoter functions in Pv11 cells as an appropriate minimal promoter with the Tet-On inducible expression system. The AcGFP1 (Aequorea coerulescens green fluorescent protein) was also successfully expressed in Pv11 cells using the inducible system. In addition to these reporter genes, the avian myeloblastosis virus reverse transcriptase α subunit (AMV RTα), which is one of the most widely commercially available RNA-dependent DNA polymerases, was successfully expressed through the inducible expression system and its catalytic activity was verified. These results demonstrate the establishment of an inducible expression system in cells that can be preserved in the dry state and highlight a possible application to the production of large and complex proteins.
Starting Page	781
e-ISSN	20754450
DOI	10.3390/insects11110781
Journal	Insects
Issue Number	11
Volume Number	11
Language	English
Publisher	MDPI
Publisher Date	2020-11-11
Access Restriction	Open
Subject Keyword	Insects Plant Sciences Anhydrobiosis Desiccation Tolerance Polypedilum Vanderplanki Insect Cells Minimal Promoter Tet-on System
Content Type	Text
Resource Type	Article

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