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A Thermocycler Using a Chip Resistor Heater and a Glass Microchip for a Portable and Rapid Microchip-Based PCR Device
| Content Provider | MDPI |
|---|---|
| Author | Yeom, Dongsun Kim, Jeongtae Kim, Sungil Ahn, Sanghoon Choi, Jiyeon Kim, Youngwook Koo, Chiwan |
| Copyright Year | 2022 |
| Description | This study proposes a rapid and inexpensive thermocycler that enables rapid heating of samples using a thin glass chip and a cheap chip resistor to overcome the on-site diagnostic limitations of polymerase chain reaction (PCR). Microchip PCR devices have emerged to miniaturize conventional PCR systems and reduce operation time and cost. In general, PCR microchips require a thin-film heater fabricated through a semiconductor process, which is a complicated process, resulting in high costs. Therefore, this investigation substituted a general chip resistor for a thin-film heater. The proposed thermocycler consists of a compact glass microchip of 12.5 mm × 12.5 mm × 2 mm that could hold a 2 μL PCR sample and a surface-mounted chip resistor of 6432 size (6.4 mm × 3.2 mm). Improving heat transfer from the chip resistor heater to the PCR reaction chamber in the microchip was accomplished via the design and fabrication of a three-dimensional chip structure using selective laser-induced etching, a rapid prototyping technique that allowed to be embedded. The fabricated PCR microchip was combined with a thermistor temperature sensor, a blower fan, and a microcontroller. The assembled thermocycler could heat the sample at a maximum rate of 28.8 °C/s per second. When compared with a commercially available PCR apparatus running the same PCR protocol, the total PCR operating time with a DNA sample was reduced by about 20%. |
| Starting Page | 339 |
| e-ISSN | 2072666X |
| DOI | 10.3390/mi13020339 |
| Journal | Micromachines |
| Issue Number | 2 |
| Volume Number | 13 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-02-21 |
| Access Restriction | Open |
| Subject Keyword | Micromachines Polymerase Chain Reaction Glass Pcr Microchip Chip Resistor Heater Sle |
| Content Type | Text |
| Resource Type | Article |
