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A New Fluorogenic Substrate for Granzyme B Based on Fluorescence Resonance Energy Transfer
| Content Provider | MDPI |
|---|---|
| Author | Martins, Cátia D. F. Raposo, M. Manuela M. Costa, Susana P. G. |
| Copyright Year | 2020 |
| Description | The synthesis and characterization of a new fluorogenic substrate for granzyme B (GzmB) is reported. The substrate design was based on the fluorescence resonance energy transfer (FRET) principle using 5-(2′-aminoethyl)aminonaphthalene sulfonic acid (Edans) and 4-[[4′-(N,N-dimethylamino)phenyl]diazenyl]benzoic acid (Dabcyl) as a donor–acceptor pair, linked to a specific sequence for GzmB (AAD), with an additional amino acid as the anchoring point (K). The tetrapeptide was synthesized by microwave-assisted solid-phase peptide synthesis (MW-SPPS) and coupled to Dabcyl and Edans at its N- and C-termini, respectively. The obtained probe was purified by semi-preparative HPLC and characterized by NMR, UV/Vis absorption and fluorescence spectroscopy and mass spectrometry. |
| Starting Page | 6 |
| e-ISSN | 26734583 |
| DOI | 10.3390/ecsoc-24-08311 |
| Journal | Chemistry Proceedings |
| Issue Number | 1 |
| Volume Number | 3 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2020-11-14 |
| Access Restriction | Open |
| Subject Keyword | Chemistry Proceedings Analytical Chemistry Donor–acceptor Pair Fret Granzyme B Fluorescent Probes Peptides |
| Content Type | Text |
