NDLI: The Preparation of Novel $P(OEGMA-co-MEO_{2}$MA) Microgels-Based Thermosensitive Hydrogel and Its Application in Three-Dimensional Cell Scaffold

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The Preparation of Novel $P(OEGMA-co-MEO_{2}$MA) Microgels-Based Thermosensitive Hydrogel and Its Application in Three-Dimensional Cell Scaffold

Content Provider	MDPI
Author	Liu, Yang Luo, Yu-Ning Zhang, Pei Yang, Wen-Fei Zhang, Cai-Yao Yin, Yu-Li
Copyright Year	2022
Description	Thermosensitive hydrogel scaffolds have attracted particular attention in three-dimensional (3D) cell culture. It is very necessary to develop a type of thermosensitive hydrogel material with low shrinkage, and excellent biocompatibility and biodegradability. Here, five types of thermosensitive microgels with different volume phase transition temperature (VPTT) or particle sizes were first synthesized using 2-methyl-2-propenoic acid-2-(2-methoxyethoxy) ethyl ester $(MEO_{2}$MA) and oligoethylene glycol methyl ether methacrylate (OEGMA) as thermosensitive monomers by free radical polymerization. Their VPTT and particle sizes were investigated by a nanometer particle size meter and an ultraviolet spectrophotometer. The feasibility of using these $P(OEGMA-co-MEO_{2}$MA) microgels to construct thermosensitive hydrogel by means of the thermal induction method is discussed for the first time. The prepared thermosensitive hydrogel with the optimum performance was screened for in situ embedding and three-dimensional (3D) culture of MCF-7 breast cancer cells. The experimental results of AO/EB and MTT methods indicate that the pioneering scaffold material has prominent biocompatibility, and cells grow rapidly in the 3D scaffold and maintain high proliferative capacity. At the same time, there is also a tendency to aggregate to form multicellular spheres. Therefore, this original $P(OEGMA-co-MEO_{2}$MA) thermosensitive hydrogel can serve as a highly biocompatible and easily functionalized 3D cell culture platform with great potential in the biomedical area.
Starting Page	313
e-ISSN	23102861
DOI	10.3390/gels8050313
Journal	Gels
Issue Number	5
Volume Number	8
Language	English
Publisher	MDPI
Publisher Date	2022-05-19
Access Restriction	Open
Subject Keyword	Gels Thermosensitive Hydrogel P(oegma-co-meo2ma) Microgel Scaffold 3d Culture
Content Type	Text
Resource Type	Article

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