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Rapid Detection of Multiple Classes of β-Lactam Antibiotics in Blood Using an NDM-1 Biosensing Assay
| Content Provider | MDPI |
|---|---|
| Author | Meng, Qinglai Wang, Yao Long, Yali Yue, Aiping Mecklenburg, Michael Tian, Shuaiyan Fu, Yujia Yao, Xiangyu Liu, Jianyi Song, Dewei Wu, Changxin Xie, Bin |
| Copyright Year | 2021 |
| Description | Currently, assays for rapid therapeutic drug monitoring (TDM) of β-lactam antibiotics in blood, which might be of benefit in optimizing doses for treatment of critically ill patients, remain challenging. Previously, we developed an assay for determining the penicillin-class antibiotics in blood using a thermometric penicillinase biosensor. The assay eliminates sample pretreatment, which makes it possible to perform semicontinuous penicillin determinations in blood. However, penicillinase has a narrow substrate specificity, which makes it unsuitable for detecting other classes of β-lactam antibiotics, such as cephalosporins and carbapenems. In order to assay these classes of clinically useful antibiotics, a novel biosensor was developed using New Delhi metallo-β-lactamase-1 (NDM-1) as the biological recognition layer. NDM-1 has a broad specificity range and is capable of hydrolyzing all classes of β-lactam antibiotics in high efficacy with the exception of monobactams. In this study, we demonstrated that the NDM-1 biosensor was able to quantify multiple classes of β-lactam antibiotics in blood plasma at concentrations ranging from 6.25 mg/L or 12.5 mg/L to 200 mg/L, which covered the therapeutic concentration windows of the tested antibiotics used to treat critically ill patients. The detection of ceftazidime and meropenem was not affected by the presence of the β-lactamase inhibitors avibactam and vaborbactam, respectively. Furthermore, both free and protein-bound β-lactams present in the antibiotic-spiked plasma samples were detected by the NDM-1 biosensor. These results indicated that the NDM-1 biosensor is a promising technique for rapid TDM of total β-lactam antibiotics present in the blood of critically ill patients. |
| Starting Page | 1110 |
| e-ISSN | 20796382 |
| DOI | 10.3390/antibiotics10091110 |
| Journal | Antibiotics |
| Issue Number | 9 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-09-14 |
| Access Restriction | Open |
| Subject Keyword | Antibiotics Microbiology Β-lactam Antibiotics Therapeutic Drug Monitoring Critically Ill Patients Thermometric Biosensor Ndm-1 |
| Content Type | Text |
| Resource Type | Article |
