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Antibacterial Activity of $TiO_{2}$ Nanoparticles Prepared by One-Step Laser Ablation in Liquid
| Content Provider | MDPI |
|---|---|
| Author | Khashan, Khawla Sulaiman, Ghassan Abdulameer, Farah Albukhaty, Salim Ibrahem, Mohammed Al-Muhimeed, Tahani AlObaid, Abeer |
| Copyright Year | 2021 |
| Description | Laser ablation in liquid was utilized to prepare a $TiO_{2}$ NP suspension in in deionized distilled water using Q-switch Nd: YAG laser at various laser energies and ablation times. The samples were characterized using UV–visible absorption spectra obtained with a UV–visible spectrophotometer (UV-Vis,) Fourier transform infrared (FTIR), X-ray diffraction (XRD), and transmission electron microscope (TEM). While, UV-Vis spectra showed the characteristic band-to-band absorption peak of $TiO_{2}$ NPs in the UV range. FTIR analysis showed the existence of O-Ti-O bond. XRD patterns indicated the presence of (101) and (112) plane crystalline phases of $TiO_{2}$. TEM images showed a spherical-like structure of $TiO_{2}$ NPs with various size distributions depending on the ablation period. It was also found that there is a relationship between laser ablation time and $TiO_{2}$ NP size distribution, where longer ablation times led to the smaller size distribution. The antibacterial activity of $TiO_{2}$ NPs was evaluated with different species of bacteria such as Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, and Staphylococcus aureus, using the liquid approach. The optimum activity of $TiO_{2}$ NPs is found to be against E. coli at 1000 μg $mL^{−1}$. Furthermore, adding, $TiO_{2}$ NPs (1000 μg $mL^{−1}$) in the presence of amoxicillin has a synergic effect on E. coli and S. aureus growth, as measured by the well diffusion method. However, both E. coli (11.6 ± 0.57mm) and S. aureus (13.3 ± 0.57mm) were inhibited by this process. |
| Starting Page | 4623 |
| e-ISSN | 20763417 |
| DOI | 10.3390/app11104623 |
| Journal | Applied Sciences |
| Issue Number | 10 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-05-19 |
| Access Restriction | Open |
| Subject Keyword | Applied Sciences Spectroscopy Tio2 Nps Laser Ablation in Liquid Characterization Antibacterial Activity |
| Content Type | Text |
| Resource Type | Article |
