NDLI: Adipose-Derived Stem Cells from Type 2 Diabetic Rats Retain Positive Effects in a Rat Model of Erectile Dysfunction

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Adipose-Derived Stem Cells from Type 2 Diabetic Rats Retain Positive Effects in a Rat Model of Erectile Dysfunction

Content Provider	MDPI
Author	Quaade, Marlene Louise Dhumale, Pratibha Steffensen, Simon Gabriel Comerma Beck, Hans Christian Harvald, Eva Bang Jensen, Charlotte Harken Lund, Lars Andersen, Ditte Caroline Sheikh, Søren Paludan
Copyright Year	2022
Description	Erectile dysfunction is a common complication associated with type 2 diabetes mellitus (T2DM) and after prostatectomy in relation to cancer. The regenerative effect of cultured adipose-derived stem cells (ASCs) for ED therapy has been documented in multiple preclinical trials as well as in recent Pase 1 trials in humans. However, some studies indicate that diabetes negatively affects the mesenchymal stem cell pool, implying that ASCs from T2DM patients could have impaired regenerative capacity. Here, we directly compared ASCs from age-matched diabetic Goto–Kakizaki $(ASC_{GK}$) and non-diabetic wild type rats $(ASC_{WT}$) with regard to their phenotypes, proteomes and ability to rescue ED in normal rats. Despite $ASC_{GK}$ exhibiting a slightly lower proliferation rate, $ASC_{GK}$ and $ASC_{WT}$ proteomes were more or less identical, and after injections to corpus cavernosum they were equally efficient in restoring erectile function in a rat ED model entailing bilateral nerve crush injury. Moreover, molecular analysis of the corpus cavernosum tissue revealed that both $ASC_{GK}$ and $ASC_{WT}$ treated rats had increased induction of genes involved in recovering endothelial function. Thus, our finding argues that T2DM does not appear to be a limiting factor for autologous adipose stem cell therapy when correcting for ED.
Starting Page	1692
e-ISSN	14220067
DOI	10.3390/ijms23031692
Journal	International Journal of Molecular Sciences
Issue Number	3
Volume Number	23
Language	English
Publisher	MDPI
Publisher Date	2022-02-01
Access Restriction	Open
Subject Keyword	International Journal of Molecular Sciences Cell Tissue Engineering Erectile Dysfunction Adipose Derived Stem/stromal Cells Type 2 Diabetes Mellitus Bilateral Nerve Crush Injury
Content Type	Text
Resource Type	Article

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