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New Biocalcifying Marine Bacterial Strains Isolated from Calcareous Deposits and Immediate Surroundings
| Content Provider | MDPI |
|---|---|
| Author | Val, érie Sopéna Sophie, Sablé Vincent, Julia Colin, Béatrice Lanneluc, Isabelle Sabot, René Turcry, Philippe Mahieux, Pierre-Yves Refait, Philippe Jeannin, Marc |
| Copyright Year | 2021 |
| Description | Marine bacterial biomineralisation by $CaCO_{3}$ precipitation provides natural limestone structures, like beachrocks and stromatolites. Calcareous deposits can also be abiotically formed in seawater at the surface of steel grids under cathodic polarisation. In this work, we showed that this mineral-rich alkaline environment harbours bacteria belonging to different genera able to induce $CaCO_{3}$ precipitation. We previously isolated 14 biocalcifying marine bacteria from electrochemically formed calcareous deposits and their immediate environment. By microscopy and µ-Raman spectroscopy, these bacterial strains were shown to produce calcite-type $CaCO_{3}$. Identification by 16S rDNA sequencing provided between 98.5 and 100% identity with genera Pseudoalteromonas, Pseudidiomarina, Epibacterium, Virgibacillus, Planococcus, and Bhargavaea. All 14 strains produced carbonic anhydrase, and six were urease positive. Both proteins are major enzymes involved in the biocalcification process. However, this does not preclude that one or more other metabolisms could also be involved in the process. In the presence of urea, Virgibacillus halodenitrificans CD6 exhibited the most efficient precipitation of $CaCO_{3}$. However, the urease pathway has the disadvantage of producing ammonia, a toxic molecule. We showed herein that different marine bacteria could induce $CaCO_{3}$ precipitation without urea. These bacteria could then be used for eco-friendly applications, e.g., the formation of bio-cements to strengthen dikes and delay coastal erosion. |
| Starting Page | 76 |
| e-ISSN | 20762607 |
| DOI | 10.3390/microorganisms10010076 |
| Journal | Microorganisms |
| Issue Number | 1 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-12-30 |
| Access Restriction | Open |
| Subject Keyword | Microorganisms Environmental Engineering Microbiology Biocalcifying Marine Bacteria Calcite Urease Carbonic Anhydrase Virgibacillus Pseudoalteromonas Pseudidiomarina Epibacterium Planococcus Bhargavaea |
| Content Type | Text |
| Resource Type | Article |
