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Antiviral, Cytotoxic, and Antioxidant Activities of Three Edible Agaricomycetes Mushrooms: Pleurotus columbinus, Pleurotus sajor-caju, and Agaricus bisporus
| Content Provider | MDPI |
|---|---|
| Author | Elhusseiny, Shaza El-Mahdy, Taghrid Awad, Mohamed Elleboudy, Nooran Farag, Mohamed Aboshanab, Khaled Yassien, Mahmoud |
| Copyright Year | 2021 |
| Description | In this study, we investigated aqueous extracts of three edible mushrooms: Agaricus bisporus (white button mushroom), Pleurotus columbinus (oyster mushroom), and Pleurotus sajor-caju (grey oyster mushroom). The extracts were biochemically characterized for total carbohydrate, phenolic, flavonoid, vitamin, and protein contents besides amino acid analysis. Triple TOF proteome analysis showed 30.1% similarity between proteomes of the two Pleurotus spp. All three extracts showed promising antiviral activities. While Pleurotus columbinus extract showed potent activity against adenovirus (Ad7, selectivity index (SI) = 4.2), Agaricus bisporus showed strong activity against herpes simplex II (HSV-2; SI = 3.7). The extracts showed low cytotoxicity against normal human peripheral blood mononuclear cells (PBMCs) and moderate cytotoxicity against prostate (PC3, DU-145); colorectal (Colo-205); cecum carcinoma (LS-513); liver carcinoma (HepG2); cervical cancer (HeLa); breast adenocarcinoma (MDA-MB-231 and MCF-7) as well as leukemia (CCRF-CEM); acute monocytic leukemia (THP1); acute promyelocytic leukemia (NB4); and lymphoma (U937) cell lines. Antioxidant activity was evaluated using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging, 2,2′-Azinobis-(3-Ethylbenzthiazolin-6-Sulfonic Acid) ABTS radical cation scavenging, and oxygen radical absorbance capacity (ORAC) assays. The three extracts showed potential antioxidant activities with the maximum activity recorded for Pleurotus columbinus $(IC_{50}$ µg/mL) = 35.13 ± 3.27 for DPPH, 13.97 ± 4.91 for ABTS, and 29.42 ± 3.21 for ORAC assays. |
| Starting Page | 645 |
| e-ISSN | 2309608X |
| DOI | 10.3390/jof7080645 |
| Journal | Journal of Fungi |
| Issue Number | 8 |
| Volume Number | 7 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-08-08 |
| Access Restriction | Open |
| Subject Keyword | Journal of Fungi Integrative and Complementary Medicine White Rot Fungus Pleurotus Columbinus Pleurotus Sajor-caju Agaricus Bisporus Medicinal Mushrooms Antioxidant Cytotoxic Antiviral |
| Content Type | Text |
| Resource Type | Article |
