NDLI: Cancer-Associated Fibroblast Subgroups Showing Differential Promoting Effect on HNSCC Progression

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Cancer-Associated Fibroblast Subgroups Showing Differential Promoting Effect on HNSCC Progression

Content Provider	MDPI
Author	Kang, Soo Hyun Oh, Su Young Lee, Heon-Jin Kwon, Tae-Geon Kim, Jin-Wook Lee, Sung-Tak Choi, So-Young Hong, Su-Hyung
Copyright Year	2021
Description	Background: The critical effect of the tumor microenvironment on cancer progression is well recognized. Recent research suggests that the cancer-promoting properties of the tumor stroma may be attributed to fibroblasts. However, the effect of cancer-associated fibroblast (CAF) on the progression of head and neck squamous cell carcinoma (HNSCC) is not well known. Methods: From the immunohistochemical analysis of head and neck squamous cell carcinoma (HNSCC) tissues, we divided CAF into two groups depending on the presence or absence of a well-demarcated boundary between epithelial cancer cells and the surrounding extracellular matrix (ECM). Primary culture of CAF was performed, followed by co-transplantation with HNSCC cells into mice oral mucosa, and the tumorigenesis was compared. The mRNA expression patterns between these two CAF groups were compared using DNA microarray analysis. Results: CAFs from cancer tissues that showed no demarcation between ECM and epithelial cancer cells (CAF-Promote) tended to stimulate Matrigel invasion of HNSCC cells. Conversely, CAFs from cancer tissues that showed a boundary with epithelial cancer cells (CAF-Delay) caused no remarkable increase in Matrigel invasion. Compared with CAF-P, CAF-D is less effective in promoting FaDu tumorigenicity in the mouse model. In DNA microarray analysis, COL3A1 and COL6A6 showed particularly high expression in the CAF-D group. Conclusions: These cancer stroma-derived collagen proteins might delay the HNSCC progression. These findings are expected to provide vital information for predicting HNSCC prognosis and developing drug targets in the future.
Starting Page	654
e-ISSN	20726694
DOI	10.3390/cancers13040654
Journal	Cancers
Issue Number	4
Volume Number	13
Language	English
Publisher	MDPI
Publisher Date	2021-02-06
Access Restriction	Open
Subject Keyword	Cancers Oncology Head and Neck Squamous Cell Carcinoma Cancer-associated Fibroblast Dna Microarray Collagen
Content Type	Text
Resource Type	Article

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