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Siphon-Controlled Automation on a Lab-on-a-Disc Using Event-Triggered Dissolvable Film Valves
| Content Provider | MDPI |
|---|---|
| Author | Jens, Ducrée Henderson, Brian Kinahan, David Rio, Jeanne Mishra, Rohit King, Damien Torres-Delgado, Sarai Mager, Dario Korvink, Jan |
| Copyright Year | 2021 |
| Description | Within microfluidic technologies, the centrifugal microfluidic “Lab-on-a-Disc” (LoaD) platform offers great potential for use at the PoC and in low-resource settings due to its robustness and the ability to port and miniaturize ‘wet bench’ laboratory protocols. We present the combination of ‘event-triggered dissolvable film valves’ with a centrifugo-pneumatic siphon structure to enable control and timing, through changes in disc spin-speed, of the release and incubations of eight samples/reagents/wash buffers. Based on these microfluidic techniques, we integrated and automated a chemiluminescent immunoassay for detection of the CVD risk factor marker C-reactive protein displaying a limit of detection (LOD) of 44.87 ng mL−1 and limit of quantitation (LoQ) of 135.87 ng mL−1. |
| Starting Page | 73 |
| e-ISSN | 20796374 |
| DOI | 10.3390/bios11030073 |
| Journal | Biosensors |
| Issue Number | 3 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-03-06 |
| Access Restriction | Open |
| Subject Keyword | Biosensors Analytical Chemistry Lab-on-a-chip Centrifugal Microfluidics Lab-on-a-disc Siphon Valves Cardiovascular Disease Chemiluminescence Immunoassay |
| Content Type | Text |
| Resource Type | Article |
