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Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?
| Content Provider | MDPI |
|---|---|
| Author | Soroka, Marianna Wasowicz, Barbara Rymaszewska, Anna |
| Copyright Year | 2021 |
| Description | In 1998, when the PCR technique was already popular, a Japanese company called Eiken Chemical Co., Ltd. designed a method known as the loop-mediated isothermal amplification of DNA (LAMP). The method can produce up to $10^{9}$ copies of the amplified DNA within less than an hour. It is also highly specific due to the use of two to three pairs of primers (internal, external, and loop), which recognise up to eight specific locations on the DNA or RNA targets. Furthermore, the Bst DNA polymerase most used in LAMP shows a high strand displacement activity, which eliminates the DNA denaturation stage. One of the most significant advantages of LAMP is that it can be conducted at a stable temperature, for instance, in a dry block heater or an incubator. The products of LAMP can be detected much faster than in standard techniques, sometimes only requiring analysis with the naked eye. The following overview highlights the usefulness of LAMP and its effectiveness in various fields; it also considers the superiority of LAMP over PCR and presents RT-LAMP as a rapid diagnostic tool for SARS-CoV-2. |
| Starting Page | 1931 |
| e-ISSN | 20734409 |
| DOI | 10.3390/cells10081931 |
| Journal | Cells |
| Issue Number | 8 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-07-29 |
| Access Restriction | Open |
| Subject Keyword | Cells Lamp Method Isothermal Amplification Sars-cov-2 Detection |
| Content Type | Text |
| Resource Type | Article |
