Loading...
Please wait, while we are loading the content...
Similar Documents
Tal2c Activates the Expression of $OsF3H_{04g}$ to Promote Infection as a Redundant TALE of Tal2b in Xanthomonas oryzae pv. oryzicola
| Content Provider | MDPI |
|---|---|
| Author | Wu, Tao Zhang, Haimiao Bi, Yunya Yu, Yue Liu, Haifeng Yang, Hong Yuan, Bin Ding, Xinhua Chu, Zhaohui |
| Copyright Year | 2021 |
| Description | Xanthomonas oryzae delivers transcription activator-like effectors (TALEs) into plant cells to facilitate infection. Following economic principles, the redundant TALEs are rarely identified in Xanthomonas. Previously, we identified the Tal2b, which activates the expression of the rice 2-oxoglutarate-dependent dioxygenase gene $OsF3H_{03g}$ to promote infection in the highly virulent strain of X. oryzae pv. oryzicola HGA4. Here, we reveal that another clustered TALE, Tal2c, also functioned as a virulence factor to target rice $OsF3H_{04g}$, a homologue of $OsF3H_{03g}$. Transferring Tal2c into RS105 induced expression of $OsF3H_{04g}$ to coincide with increased susceptibility in rice. Overexpressing $OsF3H_{04g}$ caused higher susceptibility and less salicylic acid (SA) production compared to wild-type plants. Moreover, CRISPR–Cas9 system-mediated editing of the effector-binding element in the promoters of $OsF3H_{03g}$ or $OsF3H_{04g}$ was found to specifically enhance resistance to Tal2b- or Tal2c-transferring strains, but had no effect on resistance to either RS105 or HGA4. Furthermore, transcriptome analysis revealed that several reported SA-related and defense-related genes commonly altered expression in $OsF3H_{04g}$ overexpression line compared with those identified in $OsF3H_{03g}$ overexpression line. Overall, our results reveal a functional redundancy mechanism of pathogenic virulence in Xoc in which tandem Tal2b and Tal2c specifically target homologues of host genes to interfere with rice immunity by reducing SA. |
| Starting Page | 13628 |
| e-ISSN | 14220067 |
| DOI | 10.3390/ijms222413628 |
| Journal | International Journal of Molecular Sciences |
| Issue Number | 24 |
| Volume Number | 22 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-12-20 |
| Access Restriction | Open |
| Subject Keyword | International Journal of Molecular Sciences Plant Sciences Rice Bacterial Leaf Streak Disease Resistance Tales 2ogd Xanthomonas Oryzae |
| Content Type | Text |
| Resource Type | Article |
