NDLI: Physicochemical Properties and Biocompatibility of Electrospun Polycaprolactone/Gelatin Nanofibers

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Physicochemical Properties and Biocompatibility of Electrospun Polycaprolactone/Gelatin Nanofibers

Content Provider	MDPI
Author	Lim, Wei Chowdhury, Shiplu Ng, Min Law, Jia
Copyright Year	2021
Description	Tissue-engineered substitutes have shown great promise as a potential replacement for current tissue grafts to treat tendon/ligament injury. Herein, we have fabricated aligned polycaprolactone (PCL) and gelatin (GT) nanofibers and further evaluated their physicochemical properties and biocompatibility. PCL and GT were mixed at a ratio of 100:0, 70:30, 50:50, 30:70, 0:100, and electrospun to generate aligned nanofibers. The PCL/GT nanofibers were assessed to determine the diameter, alignment, water contact angle, degradation, and surface chemical analysis. The effects on cells were evaluated through Wharton’s jelly-derived mesenchymal stem cell (WJ-MSC) viability, alignment and tenogenic differentiation. The PCL/GT nanofibers were aligned and had a mean fiber diameter within 200–800 nm. Increasing the GT concentration reduced the water contact angle of the nanofibers. GT nanofibers alone degraded fastest, observed only within 2 days. Chemical composition analysis confirmed the presence of PCL and GT in the nanofibers. The WJ-MSCs were aligned and remained viable after 7 days with the PCL/GT nanofibers. Additionally, the PCL/GT nanofibers supported tenogenic differentiation of WJ-MSCs. The fabricated PCL/GT nanofibers have a diameter that closely resembles the native tissue’s collagen fibrils and have good biocompatibility. Thus, our study demonstrated the suitability of PCL/GT nanofibers for tendon/ligament tissue engineering applications.
Starting Page	4764
e-ISSN	16604601
DOI	10.3390/ijerph18094764
Journal	International Journal of Environmental Research and Public Health
Issue Number	9
Volume Number	18
Language	English
Publisher	MDPI
Publisher Date	2021-04-29
Access Restriction	Open
Subject Keyword	International Journal of Environmental Research and Public Health Cell Tissue Engineering Polycaprolactone Gelatin Tendon Ligament Tissue Engineering Electrospinning Aligned Nanofibers
Content Type	Text
Resource Type	Article

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