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Protective and Therapeutic Effects of an IL-15:IL-15Rα-Secreting Cell-Based Cancer Vaccine Using a Baculovirus System
| Content Provider | MDPI |
|---|---|
| Author | Do-Thi, Van Anh Lee, Hayyoung Jeong, Hye Jin Lee, Jie-Oh Kim, Young Sang |
| Copyright Year | 2021 |
| Description | This study reports the use of the BacMam system to deliver and express self-assembling IL-15 and IL-15Rα genes to murine B16F10 melanoma and CT26 colon cancer cells. BacMam-based IL-15 and IL-15Rα were well-expressed and assembled to form the biologically functional IL-15:IL-15Rα complex. Immunization with this IL-15:IL-15Rα cancer vaccine delayed tumor growth in mice by inducing effector memory $CD4^{+}$ and $CD8^{+}$ cells and effector NK cells which are tumor-infiltrating. It caused strong antitumor immune responses of $CD8^{+}$ effector cells in a tumor-antigen specific manner both in vitro and in vivo and significantly attenuated Treg cells which a control virus-infected cancer vaccine could induce. Post-treatment with this cancer vaccine after a live cancer cell injection also prominently delayed the growth of the tumor. Collectively, we demonstrate a vaccine platform consisting of BacMam virus-infected B16F10 or CT26 cancer cells that secrete IL-15:IL-15Rα. This study is the first demonstration of a functionally competent soluble IL-15:IL-15Rα complex-related cancer vaccine using a baculovirus system and advocates that the BacMam system can be used as a secure and rapid method of producing a protective and therapeutic cancer vaccine. |
| Starting Page | 4039 |
| e-ISSN | 20726694 |
| DOI | 10.3390/cancers13164039 |
| Journal | Cancers |
| Issue Number | 16 |
| Volume Number | 13 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-08-11 |
| Access Restriction | Open |
| Subject Keyword | Cancers Oncology Interleukin 15 Interleukin 15rα B16f10 Melanoma Ct-26 Cytokine Anticancer |
| Content Type | Text |
| Resource Type | Article |
