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Point-of-Care PCR Assays for COVID-19 Detection
| Content Provider | MDPI |
|---|---|
| Author | O’Riordan, Alan Gupta, Niharika Augustine, Shine Narayan, Tarun Das, Asmita Kumar, D. Luong, John Malhotra, Bansi |
| Copyright Year | 2021 |
| Description | Molecular diagnostics has been the front runner in the world’s response to the COVID-19 pandemic. Particularly, reverse transcriptase-polymerase chain reaction (RT-PCR) and the quantitative variant (qRT-PCR) have been the gold standard for COVID-19 diagnosis. However, faster antigen tests and other point-of-care (POC) devices have also played a significant role in containing the spread of SARS-CoV-2 by facilitating mass screening and delivering results in less time. Thus, despite the higher sensitivity and specificity of the RT-PCR assays, the impact of POC tests cannot be ignored. As a consequence, there has been an increased interest in the development of miniaturized, high-throughput, and automated PCR systems, many of which can be used at point-of-care. This review summarizes the recent advances in the development of miniaturized PCR systems with an emphasis on COVID-19 detection. The distinct features of digital PCR and electrochemical PCR are detailed along with the challenges. The potential of CRISPR/Cas technology for POC diagnostics is also highlighted. Commercial RT–PCR POC systems approved by various agencies for COVID-19 detection are discussed. |
| Starting Page | 141 |
| e-ISSN | 20796374 |
| DOI | 10.3390/bios11050141 |
| Journal | Biosensors |
| Issue Number | 5 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-05-01 |
| Access Restriction | Open |
| Subject Keyword | Biosensors Analytical Chemistry Polymerase Chain Reaction Covid-19 Electrochemical Digital Pcr Point-of-care |
| Content Type | Text |
| Resource Type | Article |
