NDLI: Metabolic Profiling of Rumen Fluid and Milk in Lactating Dairy Cattle Influenced by Subclinical Ketosis Using Proton Nuclear Magnetic Resonance Spectroscopy

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Metabolic Profiling of Rumen Fluid and Milk in Lactating Dairy Cattle Influenced by Subclinical Ketosis Using Proton Nuclear Magnetic Resonance Spectroscopy

Content Provider	MDPI
Author	Eom, Jun-Sik Kim, Hyun-Sang Lee, Shin-Ja Choi, You-Young Jo, Seong-Uk Kim, Jaemin Lee, Sang-Suk Kim, Eun-Tae Lee, Sung-Sill
Copyright Year	2021
Description	Ketosis metabolic research on lactating dairy cattle has been conducted worldwide; however, there have been very few Korean studies. Biofluids from lactating dairy cattle are necessary to study ketosis metabolic diseases. Six Holstein cows were divided into two groups (healthy (CON) and subclinical ketosis diagnosed (SCK)). Rumen fluid and milk samples were collected using a stomach tube and a pipeline milking system, respectively. Metabolites were determined using proton nuclear magnetic resonance (NMR) spectroscopy and they were identified and quantified using the Chenomx NMR Suite 8.4 software and Metaboanalyst 5.0. In the rumen fluid of the SCK group, butyrate, sucrose, 3-hydroxybutyrate, maltose, and valerate levels were significantly higher than in the CON group, which showed higher levels of N,N-dimethylformamide, acetate, glucose, and propionate were significantly higher. Milk from the SCK group showed higher levels of maleate, 3-hydroxybutyrate, acetoacetate, galactonate, and 3-hydroxykynurenine than that from the CON group, which showed higher levels of galactitol, 1,3-dihydroxyacetone, γ-glutamylphenylalanine, 5-aminolevulinate, acetate, and methylamine. Some metabolites are associated with ketosis diseases and the quality of rumen fluid and milk. This report will serve as a future reference guide for ketosis metabolomics studies in Korea.
Starting Page	2526
e-ISSN	20762615
DOI	10.3390/ani11092526
Journal	Animals
Issue Number	9
Volume Number	11
Language	English
Publisher	MDPI
Publisher Date	2021-08-27
Access Restriction	Open
Subject Keyword	Animals Biochemical Research Lactating Dairy Cattle Metabolites Milk Rumen Fluid Subclinical Ketosis 1h-nmr Spectroscopy
Content Type	Text
Resource Type	Article

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