Loading...
Please wait, while we are loading the content...
Similar Documents
PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties
| Content Provider | MDPI |
|---|---|
| Author | Maja, Antunović Marica, Ivanković Hrvoje, Ivanković Bauer, Leonard Gallego-Ferrer, Gloria |
| Copyright Year | 2021 |
| Description | Ionic substitutions within the hydroxyapatite lattice are a widely used approach to mimic the chemical composition of the bone mineral. In this work, Sr-substituted and Mg- and Sr-co-substituted calcium phosphate (CaP) scaffolds, with various levels of strontium and magnesium substitution, were prepared using the hydrothermal method at 200 °C. Calcium carbonate skeletons of cuttlefish bone, ammonium dihydrogenphosphate $(NH_{4}H_{2}PO_{4}$), strontium nitrate $(Sr(NO_{3})_{2}$), and magnesium perchlorate $(Mg(ClO_{4})_{2}$) were used as reagents. Materials were characterized by means of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Whole powder pattern decomposition refinements of XRD data indicated that increased magnesium content in the Mg- and Sr-co-substituted scaffolds was related to an increased proportion of the whitlockite (WH) phase in the biphasic hydroxyapatite (HAp)/WH scaffolds. In addition, refinements indicate that $Sr^{2+}$ ions have replaced $Ca^{2+}$ sites in the WH phase. Furthermore, PCL-coated Mg-substituted and Sr- and Mg-co-substituted scaffolds, with the HAp:WH wt. ratio of 90:10 were prepared by vacuum impregnation. Results of compression tests showed a positive impact of the WH phase and PCL coating on the mechanical properties of scaffolds. Human mesenchymal stem cells (hMSCs) were cultured on composite scaffolds in an osteogenic medium for 21 days. Immunohistochemical staining showed that Mg-Sr-CaP/PCL scaffold exhibited higher expression of collagen type I than the Mg-CaP/PCL scaffold, indicating the positive effect of $Sr^{2+}$ ions on the differentiation of hMSCs, in concordance with histology results. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis confirmed an early stage of osteogenic differentiation. |
| Starting Page | 4403 |
| e-ISSN | 19961944 |
| DOI | 10.3390/ma14164403 |
| Journal | Materials |
| Issue Number | 16 |
| Volume Number | 14 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-08-06 |
| Access Restriction | Open |
| Subject Keyword | Materials Cell Tissue Engineering Scaffold Hydroxyapatite Whitlockite Co-substitution Poly(ε-caprolactone) Hmscs Osteogenic Differentiation |
| Content Type | Text |
| Resource Type | Article |
