NDLI: GOx/Hb Cascade Oxidized Crosslinking of Silk Fibroin for Tissue-Responsive Wound Repair

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GOx/Hb Cascade Oxidized Crosslinking of Silk Fibroin for Tissue-Responsive Wound Repair

Content Provider	MDPI
Author	Shen, Hongdou Wang, Pei Han, Xiaoke Ma, Mengli Shang, Yinghui Ju, Ye Shen, Saiji Yin, Feng Wang, Qigang
Copyright Year	2022
Description	Promising wound dressings can achieve rapid soft-tissue filling while refactoring the biochemical and biophysical microenvironment to recruit endogenous cells, facilitating tissue healing, integration, and regeneration. In this study, a tissue biomolecule-responsive hydrogel matrix, employing natural silk fibroin (SF) as a functional biopolymer and haemoglobin (Hb) as a peroxidase-like biocatalyst, was fabricated through cascade enzymatic crosslinking. The hydrogels possessed mechanical tunability and displayed adjustable gelation times. A tyrosine unit on SF stabilised the structure of Hb during the cascade oxidation process; thus, the immobilized Hb in SF hydrogels exhibited higher biocatalytic efficiency than the free enzyme system, which provided a continuously antioxidative system. The regulation of the dual enzyme ratio endowed the hydrogels with favourable biocompatibility, biodegradability, and adhesion strength. These multifunctional hydrogels provided a three-dimensional porous extracellular matrix-like microenvironment for promoting cell adhesion and proliferation. A rat model with a full-thickness skin defect revealed accelerated wound regeneration via collagen deposition, re-epithelialisation and revascularisation. Enzyme-loaded hydrogels are an attractive and high-safety biofilling material with the potential for wound healing, tissue regeneration, and haemostasis.
Starting Page	56
e-ISSN	23102861
DOI	10.3390/gels8010056
Journal	Gels
Issue Number	1
Volume Number	8
Language	English
Publisher	MDPI
Publisher Date	2022-01-12
Access Restriction	Open
Subject Keyword	Gels Silk Fibroin Antioxidative Hydrogels Cascade Oxidized Crosslinking Tissue-responsive Wound Regeneration
Content Type	Text
Resource Type	Article

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