NDLI: Development of Anti-TNFR Antibody-Conjugated Nanoparticles

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Development of Anti-TNFR Antibody-Conjugated Nanoparticles

Content Provider	MDPI
Author	Aido, Ahmed Wajant, Harald Buzgo, Matej Simaite, Aiva
Copyright Year	2020
Description	Immunotherapy is considered as a new pillar of cancer treatment. However, the application of some promising immunotherapeutic antibodies, such as antibodies against certain immune-stimulatory receptors of the TNF receptor superfamily (TNFRs), including CD40, 41BB, CD27 and anti-fibroblast growth factor-inducible 14 (anti-Fn14), are limited due to their low bioactivity. It has been previously shown that the bioactivity of such anti-TNFR antibodies could be improved by crosslinking or attachment to the plasma membrane by interaction with Fcγ receptors (FcγR). Both result in the proximity of multiple antibody-bound TNFR molecules, which allow for the activation of proinflammatory signaling pathways. In this work, we have grafted antibodies on gold nanoparticles to simulate the “activating” effect of FcγR-bound, and thus plasma membrane-presented anti-TNFR antibodies. We have developed and optimized the method for the preparation of gold nanoparticles, their functionalization with poly-ethylene glycol (PEG) linkers, and grafting of antibodies on the surface. We showed here that antibodies, including the anti-Fn14 antibody PDL192, can be successfully attached to nanoparticles without affecting antigen binding. We hypothesize that conjugation of monoclonal anti-TNFR antibodies to the inorganic nanoparticles is a promising technique to boost the efficacy of these immunotherapeutic antibodies.
Starting Page	55
e-ISSN	25043900
DOI	10.3390/IECP2020-08684
Journal	Proceedings
Issue Number	1
Volume Number	78
Language	English
Publisher	MDPI
Publisher Date	2020-12-01
Access Restriction	Open
Subject Keyword	Proceedings Biochemical Research Nanoparticles Surface Modification Drug-delivery Agonistic Anti Tnfrsf Receptor (tnfr) Antibody
Content Type	Text

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