NDLI: Identification of Two Novel Linear Neutralizing Epitopes within the Hexon Protein of Canine Adenovirus Using Monoclonal Antibodies

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Identification of Two Novel Linear Neutralizing Epitopes within the Hexon Protein of Canine Adenovirus Using Monoclonal Antibodies

Content Provider	MDPI
Author	Wang, Shujie Wang, Chunsheng Ren, Xiao Xue, Wenjiao He, Haijuan Zhu, Yanzhu Wang, Hongfeng Wang, Gang Cai, Xuehui
Copyright Year	2021
Description	Canine adenovirus (CAdV) has a high prevalence in canine populations. High affinity neutralizing antibodies against conserved epitopes can provide protective immunity against CAdV and protect against future outbreaks. In this study, we identified two CAdV-2-specific neutralizing monoclonal antibodies (mAbs), 2C1 and 7D7, which recognized two linear-dependent epitopes. MAb 2C1 potently neutralized CAdV-2 with a 50% neutralization titer (NT50) of 4096, and mAb 7D7 partially neutralized CAdV-2 with a 50% NT50 of 64. Immunoprecipitation, Western blot and protein spectral analysis indicated that both neutralizing mAbs recognized the hexon protein (Hex) of CAdV-2. Through a 12-mer random peptide phage display and synthetic peptides analysis, we finely mapped the neutralizing epitopes to two 10-amino acid (aa) peptides within the CAdV Hex: 634RIKQRETPAL643 located on the surface region; and 736PESYKDRMYS745 located in the inner region of the expected 3D structure of trimeric Hex. Importantly, the two epitopes are highly conserved among all CAdV isolates by sequence alignment analysis. Thus, these results provide insights into the interaction between virus and mAbs at the aa level and may have potential applications in the development of novel therapeutic or epitope-based vaccines, antibody therapeutics and a diagnostic method suitable for the rapid detection of all CAdVs.
Starting Page	135
e-ISSN	2076393X
DOI	10.3390/vaccines9020135
Journal	Vaccines
Issue Number	2
Volume Number	9
Language	English
Publisher	MDPI
Publisher Date	2021-02-08
Access Restriction	Open
Subject Keyword	Vaccines Immunology Virology Canine Adenovirus Identification Hexon Protein Monoclonal Antibodies Novel Epitopes
Content Type	Text
Resource Type	Article

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