Loading...
Please wait, while we are loading the content...
Similar Documents
Antimicrobial Resistance Genes and Diversity of Clones among Faecal ESBL-Producing Escherichia coli Isolated from Healthy and Sick Dogs Living in Portugal
| Content Provider | MDPI |
|---|---|
| Author | Sandra, Martínez-Álvarez Jorge, Façanha Carvalho, Isabel Cunha, Rita Martins, Carla Chenouf, Nadia Safia Pimenta, Paulo Pereira, Ana Raquel Ramos, Sónia Sadi, Madjid Martins, Ângela Rabbi, Fazle Capita, Rosa Alonso-Calleja, Carlos Dapkevicius, Maria De Lurdes Nunes Enes Igrejas, Gilberto Torres, Carmen Poeta, Patrícia |
| Copyright Year | 2021 |
| Abstract | The purpose of this study was to analyse the prevalence and genetic characteristics of ESBL and acquired-AmpC (qAmpC)-producing Escherichia coli isolates from healthy and sick dogs in Portugal. Three hundred and sixty-one faecal samples from sick and healthy dogs were seeded on MacConkey agar supplemented with cefotaxime (2 µg/mL) for cefotaxime-resistant (CTXR) E. coli recovery. Antimicrobial susceptibility testing for 15 antibiotics was performed and the ESBL-phenotype of the E. coli isolates was screened. Detection of antimicrobial resistance and virulence genes, and molecular typing of the isolates (phylogroups, multilocus-sequence-typing, and specific-ST131) were performed by PCR (and sequencing when required). CTXRE. coli isolates were obtained in 51/361 faecal samples analysed (14.1%), originating from 36/234 sick dogs and 15/127 healthy dogs. Forty-seven ESBL-producing E. coli isolates were recovered from 32 sick (13.7%) and 15 healthy animals (11.8%). Different variants of blaCTX-M genes were detected among 45/47 ESBL-producers: blaCTX-M-15 (n = 26), blaCTX-M-1 (n = 10), blaCTX-M-32 (n = 3), blaCTX-M-55 (n = 3), blaCTX-M-14 (n = 2), and blaCTX-M-variant (n = 1); one ESBL-positive isolate co-produced CTX-M-15 and CMY-2 enzymes. Moreover, two additional CTXR ESBL-negative E. coli isolates were CMY-2-producers (qAmpC). Ten different sequence types were identified (ST/phylogenetic-group/β-lactamase): ST131/B2/CTX-M-15, ST617/A/CTX-M-55, ST3078/B1/CTX-M-32, ST542/A/CTX-M-14, ST57/D/CTX-M-1, ST12/B2/CTX-M-15, ST6448/B1/CTX-M-15 + CMY-2, ST5766/A/CTX-M-32, ST115/D/CMY-2 and a new-ST/D/CMY-2. Five variants of CTX-M enzymes (CTX-M-15 and CTX-M-1 predominant) and eight different clonal complexes were detected from canine ESBL-producing E. coli isolates. Although at a lower rate, CMY-2 β-lactamase was also found. Dogs remain frequent carriers of ESBL and/or qAmpC-producing E. coli with a potential zoonotic role. |
| Starting Page | 1013 |
| e-ISSN | 20796382 |
| DOI | 10.3390/antibiotics10081013 |
| Journal | Antibiotics |
| Issue Number | 8 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-08-20 |
| Access Restriction | Open |
| Subject Keyword | Antibiotics Microbiology Antimicrobial Resistance Dogs Escherichia Coli Esbl Ctx-m-15 Ctx-m-1 Ctx-m-32 Ctx-m-55 Ctx-m-14 Qampc Cmy-2 |
| Content Type | Text |
| Resource Type | Article |
