NDLI: Capacity of Retinal Ganglion Cells Derived from Human Induced Pluripotent Stem Cells to Suppress T-Cells

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Capacity of Retinal Ganglion Cells Derived from Human Induced Pluripotent Stem Cells to Suppress T-Cells

Content Provider	MDPI
Author	Edo, Ayaka Sugita, Sunao Futatsugi, Yoko Sho, Junki Onishi, Akishi Kiuchi, Yoshiaki Takahashi, Masayo
Copyright Year	2020
Description	Retinal ganglion cells (RGCs) are impaired in patients such as those with glaucoma and optic neuritis, resulting in permanent vision loss. To restore visual function, development of RGC transplantation therapy is now underway. Induced pluripotent stem cells (iPSCs) are an important source of RGCs for human allogeneic transplantation. We therefore analyzed the immunological characteristics of iPSC-derived RGCs (iPSC-RGCs) to evaluate the possibility of rejection after RGC transplantation. We first assessed the expression of human leukocyte antigen (HLA) molecules on iPSC-RGCs using immunostaining, and then evaluated the effects of iPSC-RGCs to activate lymphocytes using the mixed lymphocyte reaction (MLR) and iPSC-RGC co-cultures. We observed low expression of HLA class I and no expression of HLA class II molecules on iPSC-RGCs. We also found that iPSC-RGCs strongly suppressed various inflammatory immune cells including activated T-cells in the MLR assay and that transforming growth factor-β2 produced by iPSC-RGCs played a critical role in suppression of inflammatory cells in vitro. Our data suggest that iPSC-RGCs have low immunogenicity, and immunosuppressive capacity on lymphocytes. Our study will contribute to predicting immune attacks after RGC transplantation.
Starting Page	7831
e-ISSN	14220067
DOI	10.3390/ijms21217831
Journal	International Journal of Molecular Sciences
Issue Number	21
Volume Number	21
Language	English
Publisher	MDPI
Publisher Date	2020-10-22
Access Restriction	Open
Subject Keyword	International Journal of Molecular Sciences Cell Tissue Engineering Retinal Ganglion Cells Induced Pluripotent Stem Cells Immunogenicity Mixed Lymphocyte Reaction Immunosuppression
Content Type	Text
Resource Type	Article

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