NDLI: Comparison of Biocompatibility of Calcium Silicate-Based Sealers and Epoxy Resin-Based Sealer on Human Periodontal Ligament Stem Cells

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Comparison of Biocompatibility of Calcium Silicate-Based Sealers and Epoxy Resin-Based Sealer on Human Periodontal Ligament Stem Cells

Content Provider	MDPI
Author	Oh, Hanseul Kim, Egan Lee, Sukjoon Park, Soyeon Chen, Dongzi Shin, Su-Jung Kim, Euiseong Kim, Sunil
Copyright Year	2020
Description	The aim of this study was to evaluate the biocompatibility of calcium silicate-based sealers (CeraSeal and EndoSeal TCS) and epoxy resin-based sealer (AH-Plus) in terms of cell viability, inflammatory response, expression of mesenchymal phenotype, osteogenic potential, cell attachment, and morphology, of human periodontal ligament stem cells (hPDLSCs). hPDLSCs were acquired from the premolars (n = 4) of four subjects, whose ages extended from 16 to 24 years of age. Flow cytometry analysis showed stemness of hPDLSCs was maintained in all materials. In cell viability test, AH-Plus showed the lowest cell viability, and CeraSeal showed significantly higher cell viability than others. In ELISA test, AH-Plus showed higher expression of IL-6 and IL-8 than calcium silicate-based sealers. In an osteogenic potential test, AH-Plus showed a lower expression level than other material; however, EndoSeal TCS showed a better expression level than others. All experiments were repeated at least three times per cell line. Scanning electronic microscopy studies showed low degree of cell proliferation on AH-Plus, and high degree of cell proliferation on calcium silicate-based sealers. In this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy-resin based sealers.
Starting Page	5242
e-ISSN	19961944
DOI	10.3390/ma13225242
Journal	Materials
Issue Number	22
Volume Number	13
Language	English
Publisher	MDPI
Publisher Date	2020-11-20
Access Restriction	Open
Subject Keyword	Materials Cell Tissue Engineering Dentistry and Oral Surgery Calcium Silicate-based Sealer Biocompatibility Root Canal Treatment Epoxy Resin-based Sealer Cell Viability Inflammatory Response Periodontal Ligament Stem Cell
Content Type	Text
Resource Type	Article

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