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Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (Camellia sinensis L. O. Kuntze) and Characterization of Amino Acid Transporter CsCAT9.1
| Content Provider | MDPI |
|---|---|
| Author | Zhang, Xinwan Liu, Hongling Pilon-Smits, Elizabeth Huang, Wei Wang, Pu Wang, Mingle Guo, Fei Wang, Yu Li, Ruiyuan Zhao, Hua Ni, Dejiang |
| Copyright Year | 2020 |
| Description | The vigor of tea plants (Camellia sinensis) and tea quality are strongly influenced by the abundance and forms of nitrogen, principally NO3−, NH4+, and amino acids. Mechanisms to access different nitrogen sources and the regulatory cues remain largely elusive in tea plants. A transcriptome analysis was performed to categorize differentially expressed genes (DEGs) in roots and young leaves during the early response to four nitrogen treatments. Relative to the continuously nitrogen-replete control, the three nitrogen-deprived and resupplied treatments shared 237 DEGs in the shoots and 21 DEGs in the root. Gene-ontology characterization revealed that transcripts encoding genes predicted to participate in nitrogen uptake, assimilation, and translocation were among the most differentially expressed after exposure to the different nitrogen regimes. Because of its high transcript level regardless of nitrogen condition, a putative amino acid transporter, TEA020444/CsCAT9.1, was further characterized in Arabidopsis and found to mediate the acquisition of a broad spectrum of amino acids, suggesting a role in amino acid uptake, transport, and deposition in sinks as an internal reservoir. Our results enhance our understanding of nitrogen-regulated transcript level patterns in tea plants and pinpoint candidate genes that function in nitrogen transport and metabolism, allowing tea plants to adjust to variable nitrogen environments. |
| Starting Page | 1218 |
| e-ISSN | 22237747 |
| DOI | 10.3390/plants9091218 |
| Journal | Plants |
| Issue Number | 9 |
| Volume Number | 9 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2020-09-17 |
| Access Restriction | Open |
| Subject Keyword | Plants Plant Sciences Assimilation Camellia Sinensis Nitrogen Transport Transcriptome |
| Content Type | Text |
| Resource Type | Article |
