NDLI: Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood

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Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood

Content Provider	MDPI
Author	Nasiri, Rohollah Shamloo, Amir Akbari, Javad
Copyright Year	2021
Description	Circulating tumor cells (CTCs) isolation from a blood sample plays an important role in cancer diagnosis and treatment. Microfluidics offers a great potential for cancer cell separation from the blood. Among the microfluidic-based methods for CTC separation, the inertial method as a passive method and magnetic method as an active method are two efficient well-established methods. Here, we investigated the combination of these two methods to separate CTCs from a blood sample in a single chip. Firstly, numerical simulations were performed to analyze the fluid flow within the proposed channel, and the particle trajectories within the inertial cell separation unit were investigated to determine/predict the particle trajectories within the inertial channel in the presence of fluid dynamic forces. Then, the designed device was fabricated using the soft-lithography technique. Later, the CTCs were conjugated with magnetic nanoparticles and Ep-CAM antibodies to improve the magnetic susceptibility of the cells in the presence of a magnetic field by using neodymium permanent magnets of 0.51 T. A diluted blood sample containing nanoparticle-conjugated CTCs was injected into the device at different flow rates to analyze its performance. It was found that the flow rate of 1000 µL/min resulted in the highest recovery rate and purity of ~95% and ~93% for CTCs, respectively.
Starting Page	877
e-ISSN	2072666X
DOI	10.3390/mi12080877
Journal	Micromachines
Issue Number	8
Volume Number	12
Language	English
Publisher	MDPI
Publisher Date	2021-07-26
Access Restriction	Open
Subject Keyword	Micromachines Analytical Chemistry Microfluidics Cell Separation Ctcs Inertial Magnetophoretic Nanoparticle
Content Type	Text
Resource Type	Article

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