NDLI: Cost-Effective Multiplex Real-Time PCR Chip System Using Open Platform Camera

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Cost-Effective Multiplex Real-Time PCR Chip System Using Open Platform Camera

Content Provider	MDPI
Author	Yun, Sung-Hun Park, Ji-Sung Koo, Seul-Bit-Na Park, Chan-Young Kim, Yu-Seop Kim, Jong-Dae
Copyright Year	2021
Description	This paper proposes a cost-effective real-time multiplexed polymerase chain reaction (PCR) chip system for point-of-care (POC) testing. In the proposed system, nucleic acid amplification is performed in a reaction chamber built on a printed-circuit-board (PCB) substrate with a PCB pattern heater and a thermistor. Fluorescence can be detected through the transparent plastic on the other side of the substrate. Open platform cameras were used for miniaturization and cost-effectiveness. We also used simple and cost-effective oblique lighting to stimulate fluorescence. Response performance was investigated by observing the change in the average brightness of the chamber images with various reference dye concentrations. In addition, we investigated the interference properties between different colors by measuring the fluorescence response for each dye concentration mixed with the maximum concentration of the different dyes. Quantitative performance was validated using standard DNA solutions. Experimental results show that the proposed system is suitable for POC real-time multi-PCR systems.
Starting Page	59
e-ISSN	26734591
DOI	10.3390/I3S2021Dresden-10071
Journal	Engineering Proceedings
Issue Number	1
Volume Number	6
Language	English
Publisher	MDPI
Publisher Date	2021-05-17
Access Restriction	Open
Subject Keyword	Engineering Proceedings Industrial Engineering Real-time Polymerase Chain Reaction Multiplexing Open Platform Camera Pcr Chip Pcb Substrate
Content Type	Text

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