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Development of Lateral Flow Immunochromatographic Test for Rapid Detection of SARS-CoV-2 Virus Antigens in Clinical Specimens
| Content Provider | MDPI |
|---|---|
| Author | Sayed, Rafik Hamed Abousenna, Mohamed Samy Elsaady, Shaimaa Abdelall Soliman, Rafik Saad, Mohamed Ahmed |
| Copyright Year | 2022 |
| Description | In the presented study, we developed a nanogold lateral glow immunoassay-based technique (LFI-COVID-19 antigen test) for the detection of SARS-CoV-2 nucleocapsid proteins; the developed LFI-COVID-19 Ag test has been tested for limit of detection (LOD), cross-reactivity and interfering substances, and performance. It was found that the performance of the developed LFI-COVID-19 antigen test when it was evaluated by RT-qPCR indicated 95, 98, and 97% for sensitivity, specificity and accuracy, respectively. This complies with the WHO guidelines. It was concluded that the developed LFI-COVID-19 antigen test is a point of care and an alternative approach to current laboratory methods, especially RT-qPCR. It provides an easy, rapid (within 20 min), and on-site diagnostic tool for COVID-19 infection, and it is a cheap test if it is manufactured on a large scale for commercial use. |
| Starting Page | 2477 |
| e-ISSN | 20794991 |
| DOI | 10.3390/nano12142477 |
| Journal | Nanomaterials |
| Issue Number | 14 |
| Volume Number | 12 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-07-19 |
| Access Restriction | Open |
| Subject Keyword | Nanomaterials Microbiology Sars-cov-2 Covid-19 Lateral Flow Assay Antigen Testing Sensitivity and Specificity Diagnostic Testing Immuno-chromatographic Gold Nanoparticles |
| Content Type | Text |
| Resource Type | Article |
