NDLI: Dynamic Expression of Membrane Type 1-Matrix Metalloproteinase (Mt1-mmp/Mmp14) in the Mouse Embryo

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Dynamic Expression of Membrane Type 1-Matrix Metalloproteinase (Mt1-mmp/Mmp14) in the Mouse Embryo

Content Provider	MDPI
Author	Emma, Muñoz-Sáez Cristina, Sánchez-Camacho Moracho, Natalia Learte, Ana I. R. Arroyo, Alicia G.
Copyright Year	2021
Description	MT1-MMP/MMP14 belongs to a subgroup of the matrix metalloproteinases family that presents a transmembrane domain, with a cytosolic tail and the catalytic site exposed to the extracellular space. Deficient mice for this enzyme result in early postnatal death and display severe defects in skeletal, muscle and lung development. By using a transgenic line expressing the LacZ reporter under the control of the endogenous Mt1-mmp promoter, we reported a dynamic spatiotemporal expression pattern for Mt1-mmp from early embryonic to perinatal stages during cardiovascular development and brain formation. Thus, Mt1-mmp shows expression in the endocardium of the heart and the truncus arteriosus by E8.5, and is also strongly detected during vascular system development as well as in endothelial cells. In the brain, LacZ reporter expression was detected in the olfactory bulb, the rostral cerebral cortex and the caudal mesencephalic tectum. LacZ-positive cells were observed in neural progenitors of the spinal cord, neural crest cells and the intersomitic region. In the limb, Mt1-mmp expression was restricted to blood vessels, cartilage primordium and muscles. Detection of the enzyme was confirmed by Western blot and immunohistochemical analysis. We suggest novel functions for this metalloproteinase in angiogenesis, endocardial formation and vascularization during organogenesis. Moreover, Mt1-mmp expression revealed that the enzyme may contribute to heart, muscle and brain throughout development.
Starting Page	2448
e-ISSN	20734409
DOI	10.3390/cells10092448
Journal	Cells
Issue Number	9
Volume Number	10
Language	English
Publisher	MDPI
Publisher Date	2021-09-17
Access Restriction	Open
Subject Keyword	Cells Pathology Mt1-mmp Mmp14 Metalloproteinases Embryo Development Angiogenesis Expression Pattern Cardiovascular Development Brain Limb Dorsal Aorta
Content Type	Text
Resource Type	Article

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